Transcriptomic and co-expression network analyses on diverse wheat landraces identifies candidate master regulators of the response to early drought.
Transcriptomic and co-expression network analyses on diverse wheat landraces identifies candidate master regulators of the response to early drought.
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DOI：
10.3389/fpls.2023.1212559
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
全世界有超过40亿人依赖于面包小麦（Triticum aestivum L.）作为他们饮食的主要成分。然而，不断变化的气候威胁着这些人的粮食安全，严重的干旱已经造成小麦产量普遍下降。许多关于小麦干旱反应的研究都集中在对发育后期、开花期或灌浆期干旱事件的反应上。但是，随着干旱压力时期的时间变得越来越不可预测，也需要更全面地了解早期发育期间对干旱的反应。在这里，我们利用YoGI landrace panel鉴定了10，199个在早期干旱胁迫下差异表达的基因，然后使用加权基因共表达网络分析（WGCNA）构建共表达网络，并鉴定了与早期干旱反应特别相关的模块中的枢纽基因。在这些枢纽基因中，有两个突出作为早期干旱反应的新候选主调节因子-一个作为激活因子（TaDHN 4-D1; TraesCS 5D 02 G379200），另一个作为抑制因子（未表征基因; TraesCS 3D 02 G361500）。除了协调转录早期干旱反应外，我们还提出，这些枢纽基因可能能够调节生理早期干旱反应，这是因为它们可能控制许多植物物种中参与干旱反应的基因家族成员的表达，即拟南芥蛋白和水通道蛋白，以及其他似乎参与关键过程的基因，如气孔开放，气孔关闭、气孔形态发生和胁迫激素信号传导。
Over four billion people around the world rely on bread wheat (Triticum aestivum L.) as a major constituent of their diet. The changing climate, however, threatens the food security of these people, with periods of intense drought stress already causing widespread wheat yield losses. Much of the research into the wheat drought response has centred on the response to drought events later in development, during anthesis or grain filling. But as the timing of periods of drought stress become increasingly unpredictable, a more complete understanding of the response to drought during early development is also needed. Here, we utilized the YoGI landrace panel to identify 10,199 genes which were differentially expressed under early drought stress, before weighted gene co-expression network analysis (WGCNA) was used to construct a co-expression network and identify hub genes in modules particularly associated with the early drought response. Of these hub genes, two stood out as novel candidate master regulators of the early drought response – one as an activator (TaDHN4-D1; TraesCS5D02G379200) and the other as a repressor (uncharacterised gene; TraesCS3D02G361500). As well as appearing to coordinate the transcriptional early drought response, we propose that these hub genes may be able to regulate the physiological early drought response due to potential control over the expression of members of gene families well-known for their involvement in the drought response in many plant species, namely dehydrins and aquaporins, as well as other genes seemingly involved in key processes such as, stomatal opening, stomatal closing, stomatal morphogenesis and stress hormone signalling.
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影响因子：
5.6
作者：
Cantalapiedra CP;García-Pereira MJ;Gracia MP;Igartua E;Casas AM;Contreras-Moreira B
通讯作者：
Contreras-Moreira B
影响因子：
4.4
作者：
Aprile A;Mastrangelo AM;De Leonardis AM;Galiba G;Roncaglia E;Ferrari F;De Bellis L;Turchi L;Giuliano G;Cattivelli L
通讯作者：
Cattivelli L
影响因子：
1.9
作者：
Smita S;Katiyar A;Pandey DM;Chinnusamy V;Archak S;Bansal KC
通讯作者：
Bansal KC
影响因子：
3.7
作者：
Boudiar, Ridha;Casas, Ana M.;Igartua, Ernesto
通讯作者：
Igartua, Ernesto
影响因子：
5.3
作者：
Beena R;Kirubakaran S;Nithya N;Manickavelu A;Sah RP;Abida PS;Sreekumar J;Jaslam PM;Rejeth R;Jayalekshmy VG;Roy S;Manju RV;Viji MM;Siddique KHM
通讯作者：
Siddique KHM