Overexpression of the Jojoba Aquaporin Gene, ScPIP1, Enhances Drought and Salt Tolerance in Transgenic Arabidopsis

Overexpression of the Jojoba Aquaporin Gene, ScPIP1, Enhances Drought and Salt Tolerance in Transgenic Arabidopsis
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荷荷巴水通道蛋白基因 ScPIP1 的过度表达增强转基因拟南芥的干旱和盐耐受性

DOI:
10.3390/ijms20010153
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发表时间:
2019-01
影响因子:
5.6
通讯作者:
Genfa Zhang
Genfa Zhang
中科院分区:
生物学2区
文献类型:
--
作者:
Genfa Zhang

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质膜内在蛋白(PIP)是位于质膜上的水通道蛋白的一个亚家族,在质膜上它们促进水和小的不带电溶质的运输。PIP在植物的整个发育过程中起着重要的作用,并在响应非生物胁迫。希蒙得木(Simmondsiahinensis(Link)Schneider)是一种典型的荒漠植物,具有耐旱、耐盐碱、耐贫瘠等特性。在这项研究中,PIP1基因(ScPIP1)克隆霍霍巴和拟南芥中的过量表达。聚乙二醇(PEG)处理诱导ScPIP1在转录水平的表达。与野生型植物相比,ScPIP 1过表达的拟南芥植物在干旱和盐胁迫下表现出更高的发芽率、更长的根和更高的存活率。丙二醛(MDA),离子渗漏(IL)和脯氨酸含量的测量结果表明,提高耐旱性和耐盐性的ScPIP1赋予与减少膜损伤和改善渗透调节。基于ScPIP1在转基因拟南芥中的抗性效应,我们推测ScPIP1可能被应用于基因工程以提高植物的耐受性。
Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1.
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