Natural infection by Zika virus but not DNA vaccination consistently elicits antibodies that compete with two potently neutralising monoclonal antibodies targeting distinct epitopes.
Natural infection by Zika virus but not DNA vaccination consistently elicits antibodies that compete with two potently neutralising monoclonal antibodies targeting distinct epitopes.
复制标题
寨卡病毒的自然感染，而不是DNA疫苗接种始终引起的抗体，这些抗体与两种有效中和靶向不同表位的单克隆抗体竞争。
DOI：
10.1016/j.ebiom.2023.104875
复制
发表时间：
2023-12
期刊：
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
Autochthonous transmission of Zika virus (ZIKV) has been reported in 87 countries since 2015. Although most infections are mild, there is risk of Guillain-Barré syndrome and adverse pregnancy outcomes. Vaccines are urgently needed to prevent Zika, but sufficient understanding of humoral responses and tools to assess ZIKV-specific immunity are lacking. We developed a blockade-of-binding (BOB) ELISA using A9E and G9E, two strongly neutralising ZIKV-specific monoclonal antibodies, which do not react with dengue virus. Receiver operating characteristic curve analysis assessed A9E and G9E BOB serodiagnostic performance. BOB was then applied to samples from a surveillance cohort in Risaralda, Colombia, and phase 1 ZIKV vaccine trial samples, comparing results against traditional serologic tests. In the validation sample set (n = 120), A9E BOB has a sensitivity of 93.5% (95% CI: 79.3, 98.9) and specificity 97.8 (95% CI: 92.2, 99.6). G9E BOB had a sensitivity of 100% (95% CI: 89.0, 100.0) and specificity 100% (95% CI: 95.9, 100). Serum from natural infections consistently tested positive in these assays for up to one year, and reactivity tracks well with ZIKV infection status among sera from endemic areas with complicated flavivirus exposures. Interestingly, a leading ZIKV vaccine candidate elicited minimal BOB reactivity despite generating neutralising antibody responses. In conclusion, A9E and G9E BOB assays are sensitive and specific assays for detecting antibodies elicited by recent or remote ZIKV infections. Given the additional ability of these BOB assays to detect immune responses that target different epitopes, further development of these assays is well justified for applications including flavivirus surveillance, translational vaccinology research and as potential serologic correlates of protective immunity against Zika. R21 AI129532 (PI: S. Becker-Dreps), BAA 2017-N-18041 (PI: A. M. de Silva), (PI: M. H. Collins), K22 AI137306 (PI: M. H. Collins).
登录
查看更多内容
影响因子：
2.2
作者：
Chen X;Anderson LJ;Rostad CA;Ding L;Lai L;Mulligan M;Rouphael N;Natrajan MS;McCracken C;Anderson EJ
通讯作者：
Anderson EJ
影响因子：
16.6
作者：
Hasan SS;Miller A;Sapparapu G;Fernandez E;Klose T;Long F;Fokine A;Porta JC;Jiang W;Diamond MS;Crowe JE Jr;Kuhn RJ;Rossmann MG
通讯作者：
Rossmann MG
DOI：
10.1093/cid/cix732
发表时间：
2018-01-06
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Lai L;Rouphael N;Xu Y;Natrajan MS;Beck A;Hart M;Feldhammer M;Feldpausch A;Hill C;Wu H;Fairley JK;Lankford-Turner P;Kasher N;Rago P;Hu YJ;Edupuganti S;Patel SM;Murray KO;Mulligan MJ;Emory Zika Patient Study Team
通讯作者：
Emory Zika Patient Study Team
影响因子：
17.1
作者：
Abbink P;Larocca RA;Visitsunthorn K;Boyd M;De La Barrera RA;Gromowski GD;Kirilova M;Peterson R;Li Z;Nanayakkara O;Nityanandam R;Mercado NB;Borducchi EN;Chandrashekar A;Jetton D;Mojta S;Gandhi P;LeSuer J;Khatiwada S;Lewis MG;Modjarrad K;Jarman RG;Eckels KH;Thomas SJ;Michael NL;Barouch DH
通讯作者：
Barouch DH
影响因子：
8
作者：
Collins, Matthew H.;Tu, Huy A.;de Silva, Aravinda M.
通讯作者：
de Silva, Aravinda M.