Preparation of Recombinant Human Monoclonal Antibody Fab Fragments Specific for Entamoeba histolytica
Preparation of Recombinant Human Monoclonal Antibody Fab Fragments Specific for Entamoeba histolytica
复制标题
溶组织内阿米巴特异性重组人单克隆抗体Fab片段的制备
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Ihara
中科院分区：
文献类型：
--
作者：
H. Tachibana;Xun Cheng;Katsuomi Watanabe;M. Takekoshi;F. Maeda;S. Aotsuka;Yoshimasa Kaneda;T. Takeuchi;S. Ihara
ABSTRACT Genes coding for human antibody Fab fragments specific forEntamoeba histolytica were cloned and expressed inEscherichia coli. Lymphocytes were separated from the peripheral blood of a patient with an amebic liver abscess. Poly(A)+ RNA was isolated from the lymphocytes, and then genes coding for the light chain and Fd region of the heavy chain were amplified by a reverse transcriptase PCR. The amplified DNA fragments were ligated with a plasmid vector and were introduced intoEscherichia coli. Three thousand colonies were screened for the production of antibodies to E. histolytica HM-1:IMSS by an indirect fluorescence-antibody (IFA) test. Lysates from fiveEscherichia coli clones were positive. Analysis of the DNA sequences of the five clones showed that three of the five heavy-chain sequences and four of the five light-chain sequences differed from each other. When the reactivities of the Escherichia colilysates to nine reference strains of E. histolytica were examined by the IFA test, three Fab fragments with different DNA sequences were found to react with all nine strains and another Fab fragment was found to react with seven strains. None of the four human monoclonal antibody Fab fragments reacted with Entamoeba dispar reference strains or with other enteric protozoan parasites. These results indicate that the bacterial expression system reported here is effective for the production of human monoclonal antibodies specific for E. histolytica. The recombinant human monoclonal antibody Fab fragments may be applicable for distinguishing E. histolytica from E. dispar and for use in the serodiagnosis of amebiasis.
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针对溶组织内阿米巴半乳糖结合凝集素的单克隆抗体可增强粘附性。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
PetriJr,WA;Snodgrass,TL;Jackson,TF;Gathiram,V;Simjee,AE;Chadee,K;Chapman,MD
通讯作者：
Chapman,MD
DOI：
10.1073/pnas.88.22.10134
发表时间：
1991-11-01
影响因子：
11.1
作者：
BURTON, DR;BARBAS, CF;LERNER, RA
通讯作者：
LERNER, RA
简短报告：直接在粪便中检测溶组织阿米巴和迪斯帕阿米巴。
DOI：
10.4269/ajtmh.1994.50.595
发表时间：
1994
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Haque,R;Neville,LM;Wood,S;PetriJr,WA
通讯作者：
PetriJr,WA
人抗阿米巴抗体可防止严重联合免疫缺陷小鼠阿米巴肝脓肿形成。
DOI：
10.4269/ajtmh.1996.55.330
发表时间：
1996
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Seydel,KB;Braun,KL;Zhang,T;Jackson,TF;StanleyJr,SL
通讯作者：
StanleyJr,SL
溶组织内阿米巴凝集素细胞质结构域（含有 β2 整联蛋白基序）对粘附和毒力的调节。
影响因子：
3.3
作者：
Vines,RR;Ramakrishnan,G;Rogers,JB;Lockhart,LA;Mann,BJ;PetriJr,WA
通讯作者：
PetriJr,WA