High-resolution mapping and digital atlas of subcortical regions in the macaque monkey based on matched MAP-MRI and histology.
High-resolution mapping and digital atlas of subcortical regions in the macaque monkey based on matched MAP-MRI and histology.
复制标题
基于匹配的MAP-MRI和组织学的猕猴皮层下区域的高分辨率映射和数字图谱。
DOI：
10.1016/j.neuroimage.2021.118759
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发表时间：
2021-12-15
期刊：
影响因子：
5.7
通讯作者：
Basser PJ
中科院分区：
文献类型：
--
作者：
Saleem KS;Avram AV;Glen D;Yen CC;Ye FQ;Komlosh M;Basser PJ
已知皮质下核和其他脑深部结构在中枢和外周神经系统的调节中起重要作用。在常规MRI中可能难以识别和描绘这些核中的许多核及其更精细的细分，这是由于它们的小尺寸、埋藏位置以及与相邻组织相比通常细微的对比度。为了解决这个问题，我们在离体非人灵长类动物（NHP）大脑中应用了多模态方法，该方法包括高分辨率平均表观传播因子（MAP）-MRI和在同一受试者的大脑中用高分辨率显微镜成像的五种不同的组织学染色。通过将这些高维MRI数据与高分辨率组织学数据进行配准，我们可以绘制猕猴大脑皮层下灰质区域的位置、边界、细分和微结构特征。在高空间分辨率下，一般的扩散MRI，特别是MAP-MRI，可以区分大量的深部脑结构，包括较大和较小的白色物质纤维束以及各种核团内的结构特征。与来自同一大脑的组织学的相关性使得能够彻底验证用MAP-MRI识别的结构。此外，在MAP-MRI参数图像中明显的解剖细节在常规T1加权图像中不可见。我们还从三维分割的MRI切片中获得了皮质下模板“SC21”，并将该体积与先前发表的具有皮质分割的解剖模板配准，从而将皮质和皮质下区域的3D分割整合到同一体积中。这个新更新的三维D99数字脑图谱（V2.0）预期用作猕猴神经解剖学、功能和连接成像研究的参考标准，涉及皮质和皮质下靶点。SC21和D99数字模板可作为标准NIFTI和GIFTI格式的体积和表面提供。
Subcortical nuclei and other deep brain structures are known to play an important role in the regulation of the central and peripheral nervous systems. It can be difficult to identify and delineate many of these nuclei and their finer subdivisions in conventional MRI due to their small size, buried location, and often subtle contrast compared to neighboring tissue. To address this problem, we applied a multi-modal approach in ex vivo non-human primate (NHP) brain that includes high-resolution mean apparent propagator (MAP)-MRI and five different histological stains imaged with high-resolution microscopy in the brain of the same subject. By registering these high-dimensional MRI data to high-resolution histology data, we can map the location, boundaries, subdivisions, and micro-architectural features of subcortical gray matter regions in the macaque monkey brain. At high spatial resolution, diffusion MRI in general, and MAP-MRI in particular, can distinguish a large number of deep brain structures, including the larger and smaller white matter fiber tracts as well as architectonic features within various nuclei. Correlation with histology from the same brain enables a thorough validation of the structures identified with MAP-MRI. Moreover, anatomical details that are evident in images of MAP-MRI parameters are not visible in conventional T1-weighted images. We also derived subcortical template “SC21” from segmented MRI slices in three-dimensions and registered this volume to a previously published anatomical template with cortical parcellation, thereby integrating the 3D segmentation of both cortical and subcortical regions into the same volume. This newly updated three-dimensional D99 digital brain atlas (V2.0) is intended for use as a reference standard for macaque neuroanatomical, functional, and connectional imaging studies, involving both cortical and subcortical targets. The SC21 and D99 digital templates are available as volumes and surfaces in standard NIFTI and GIFTI formats.
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影响因子：
2.9
作者：
Crittenden JR;Graybiel AM
通讯作者：
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影响因子：
4.8
作者：
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Avram AV;Sarlls JE;Barnett AS;Özarslan E;Thomas C;Irfanoglu MO;Hutchinson E;Pierpaoli C;Basser PJ
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3.3
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通讯作者：
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