Molecular and genealogical analysis of grain dormancy in Japanese wheat varieties, with specific focus on MOTHER OF FT AND TFL1 on chromosome 3A.
Molecular and genealogical analysis of grain dormancy in Japanese wheat varieties, with specific focus on MOTHER OF FT AND TFL1 on chromosome 3A.
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DOI：
10.1270/jsbbs.65.103
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发表时间：
2015-03
期刊：
影响因子：
2.4
通讯作者：
Nakamura S
中科院分区：
文献类型：
--
作者：
Chono M;Matsunaka H;Seki M;Fujita M;Kiribuchi-Otobe C;Oda S;Kojima H;Nakamura S
小麦(Triticum aestivum L.)‘Zenkoujikomugi’是位于3A染色体上的FT和TFL1的母本启动子上的单核苷酸多态性(SNP)，导致基因表达水平的提高，导致籽粒的强烈休眠。我们用DNA标记检测了日本小麦品种的Zenkoujikomugi(Zen-type)SNP，并检测了日本小麦品种的MFT-3A基因，发现324个品种中有169个品种携带Zen-type SNP。在日本商品品种中，Zen-type SNP的频率在日本南部显著较高，而在北部较低。为了研究MFT-3A基因与小麦休眠的关系，我们在小麦的三个生长季节进行了种子萌发试验。平均而言，携带禅型SNP的品种比携带非禅型SNP的品种表现出更强的籽粒休眠。在商品品种中，‘Iwainodaichi’(九州)、‘Junreikomugi’(Kinki-Chugoku-Shikoku)、‘Kinuhime’(关东-东海)、‘Nbarigoshi’(东北-Hokuriku)和‘Kitamoe’(北海道)表现出最强的籽粒休眠，除‘Kitamoe’外，其余品种均携带禅型SNP。近年来，东北-北陆地区携带禅宗SNP的品种数量有所增加，但北海道地区没有增加。
In the wheat (Triticum aestivum L.) cultivar ‘Zenkoujikomugi’, a single nucleotide polymorphism (SNP) in the promoter of MOTHER OF FT AND TFL1 on chromosome 3A (MFT-3A) causes an increase in the level of gene expression, resulting in strong grain dormancy. We used a DNA marker to detect the ‘Zenkoujikomugi’-type (Zen-type) SNP and examined the genotype of MFT-3A in Japanese wheat varieties, and we found that 169 of 324 varieties carry the Zen-type SNP. In Japanese commercial varieties, the frequency of the Zen-type SNP was remarkably high in the southern part of Japan, but low in the northern part. To examine the relationship between MFT-3A genotype and grain dormancy, we performed a germination assay in three wheat-growing seasons. On average, the varieties carrying the Zen-type SNP showed stronger grain dormancy than the varieties carrying the non-Zen-type SNP. Among commercial cultivars, ‘Iwainodaichi’ (Kyushu), ‘Junreikomugi’ (Kinki-Chugoku-Shikoku), ‘Kinuhime’ (Kanto-Tokai), ‘Nebarigoshi’ (Tohoku-Hokuriku), and ‘Kitamoe’ (Hokkaido) showed the strongest grain dormancy in each geographical group, and all these varieties, except for ‘Kitamoe’, were found to carry the Zen-type SNP. In recent years, the number of varieties carrying the Zen-type SNP has increased in the Tohoku-Hokuriku region, but not in the Hokkaido region.
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影响因子：
3.3
作者：
Liu, Shubing;Sehgal, Sunish K.;Bai, Guihua
通讯作者：
Bai, Guihua
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Mori, M;Uchino, N;Miura, H
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Kulwal, Pawan;Ishikawa, Goro;Sorrells, Mark E.
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Sorrells, Mark E.
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作者：
KING, RW
通讯作者：
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