Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNAs in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization.
Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNAs in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization.
复制标题
通过原位杂交证明链脲佐菌素诱导的糖尿病大鼠表面活性蛋白 SP-A、SP-B 和 SP-C mRNA 的差异表达。
DOI：
10.1165/ajrcmb.11.4.7917308
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发表时间：
1994
影响因子：
6.4
通讯作者：
T. Morioka
中科院分区：
文献类型：
--
作者：
K. Sugahara;K. Iyama;K. Sano;T. Morioka
我们先前已经证明，通过原位杂交和北方印迹分析，肺泡II型细胞和无纤毛细支气管上皮细胞（克拉拉）在糖尿病大鼠肺表面活性蛋白A（SP-A）减少，但增加的mRNA。在本研究中，我们研究了两种疏水表面活性蛋白，SP-B和SP-C的mRNA表达和定位，并将其与SP-A mRNA水平和细胞定位在链脲佐菌素诱导的糖尿病肺进行了比较。定位和表达水平进行了分析，使用类型特异性，表面活性剂cDNA探针原位杂交。通过腹腔注射60 mg/kg链脲佐菌素在成年大鼠中诱导糖尿病。注射后10周，与对照组相比，在糖尿病肺的肺泡II型细胞中观察到更高数量的代表SP-A和SP-B mRNA的银颗粒。此外，与对照组相比，糖尿病肺的肺泡II型细胞中代表SP-C mRNA的银颗粒的数量减少，并且对照组或糖尿病组的细支气管上皮细胞中不存在代表SP-C mRNA的银颗粒。相反，在糖尿病肺细支气管上皮细胞中，SP-A mRNA的银颗粒的相对丰度增加约2倍以上的控制，而SP-B mRNA略有下降。总之，与对照组相比，糖尿病肺的肺泡和细支气管上皮细胞中SP-A、SP-B和SP-C mRNA的表达水平存在差异。（250字处删节）
We have previously demonstrated by in situ hybridization and Northern blot analysis that alveolar type II cells and nonciliated bronchiolar epithelial (Clara) cells in lungs of rats with diabetes have decreased surfactant protein A (SP-A) but increased mRNA. In the present study, we have examined the mRNA expression and localization of two hydrophobic surfactant proteins, SP-B and SP-C, and have compared them with SP-A mRNA levels and cellular localization in streptozotocin-induced diabetic lungs. Localization and level of expression were analyzed by in situ hybridization using type-specific, surfactant cDNA probes. Diabetes was induced in adult rats by an intraperitoneal injection of 60 mg/kg streptozotocin. Ten weeks after injection, higher numbers of silver grains representing SP-A and SP-B mRNAs were observed in alveolar type II cells of diabetic lungs, compared with control lungs. Moreover, the number of silver grains representing SP-C mRNA decreased in alveolar type II cells from diabetic lungs compared with controls, and no silver grains for SP-C mRNA were present in bronchiolar epithelial cells from either control or diabetic groups. In contrast, in bronchiolar epithelial cells of diabetic lungs, the relative abundance of silver grains for SP-A mRNA increased approximately 2-fold above controls, while SP-B mRNA decreased slightly. Taken together, there is differential expression in the level of SP-A, SP-B, and SP-C mRNAs in both alveolar and bronchiolar epithelial cells from diabetic lungs when compared with control lungs.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
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作者：
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DOI：
10.1016/s0006-291x(87)80519-3
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DOI：
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1986
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影响因子：
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DOI：
10.1165/ajrcmb/3.3.217
发表时间：
1990
影响因子：
6.4
作者：
Miller,BE;Bakewell,WE;Katyal,SL;Singh,G;Hook,GE
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DOI：
10.1164/arrd.1987.135.3.617
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
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作者：
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