The expression and role of stromal cell-derived factor-1alpha-CXCR4 axis in human dental pulp.
The expression and role of stromal cell-derived factor-1alpha-CXCR4 axis in human dental pulp.
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DOI：
10.1016/j.joen.2008.05.015
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发表时间：
2008-08
影响因子：
4.2
通讯作者：
Ritchie, Helena H.
中科院分区：
文献类型：
--
作者：
Jiang, Long;Zhu, Ya-Qin;Du, Rong;Gu, Ying-Xin;Xia, Lie;Qin, Feng;Ritchie, Helena H.
关键词：
最近的研究表明，SDF（基质细胞衍生因子）-1α-CXCR 4轴对肌肉和神经组织损伤后修复中的干细胞和祖细胞募集具有直接影响。没有关于牙齿组织中SDF-1α或CXCR 4的信息。本研究旨在检测SDF-1α及其受体CXCR 4在健康和炎症牙髓中的表达，并探讨SDF-1α对牙髓细胞增殖和迁移的影响。免疫组化和RT-PCR检测到SDF-1α和CXCR 4在健康牙髓中弱表达，在炎症牙髓中强表达。MTT法检测SDF-1α对DPC的增殖无促进作用。然而，迁移试验表明SDF-1α增强DPC迁移，并且可以被抗CXCR 4抗体消除。综上所述，这些结果表明SDF-1α-CXCR 4轴可能在CXCR 4阳性DPC向损伤部位募集中发挥作用
Recent reports have suggested that SDF (Stromal cell-derived factor)-1α- CXCR4 axis has a direct effect on stem and progenitor cell recruitment in muscle and neural tissue repair after injury. No information is available about SDF-1α or CXCR4 in dental tissues. The aim of this study was to assess the expression of SDF-1α and its receptor, CXCR4, in healthy or inflamed human dental pulp and to evaluate the effects of SDF-1α on dental pulp cells (DPCs) in both proliferation and migration in vitro. Immunohistochemical staining and RT-PCR detected weak expression of SDF-1α and CXCR4 in healthy dental pulp and strong expression of SDF-1a and CXCR4 in inflamed dental pulp. An MTT assay demonstrated that SDF-1α could not promote DPCs proliferation. A transmigration assay, however, indicated that SDF-1α enhanced DPCs migration, and which could be abolished by anti-CXCR4 antibodies. Taken together, these results imply that the SDF-1α-CXCR4 axis may play a role in the recruitment of CXCR4-positive DPCs toward the damaged sites
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影响因子：
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作者：
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通讯作者：
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影响因子：
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