Enrichment of polycyclic aromatic hydrocarbon metabolizing microorganisms on the oral mucosa of tobacco users.
Enrichment of polycyclic aromatic hydrocarbon metabolizing microorganisms on the oral mucosa of tobacco users.
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作者：
Tao L;Chiarelli MP;Pavlova S;Kolokythas A;Schwartz J;DeFrancesco J;Salameh B;Green SJ;Adami G
某些土壤微生物抵抗并代谢多环芳烃（PAH）。对于皮肤微生物的子集也是如此。在人类口腔中，口腔微生物有可能氧化烟草多环芳烃，从而增加这些化学物质导致邻近上皮细胞癌症的能力。我们假设，我们可以确定，在吸烟者中，口腔粘膜微生物可以代谢PAH。我们分离的细菌和真菌，长期生存在最低限度的媒体与多环芳烃作为唯一的碳源，在有氧条件下，从口腔粘膜中的17个26吸烟者和两个14非吸烟者。在严酷的PAH体外暴露中存活下来的细菌属中，除了葡萄球菌属、放线菌属和金氏菌属以外，大多数都是微量的。2株PAH耐药的白色念珠菌（C.白色念珠菌）。C.白色念珠菌是烟草使用者致癌的主要候选者，因为在烟草使用者的口腔粘膜上发现了高水平的白色念珠菌，并且一些念珠菌属物种可以代谢多环芳烃。然而，当C.测试了白色念珠菌分离株对两种模型PAH底物（芘和菲）的代谢，它们不能代谢PAH，这表明它们在所用条件下不能代谢PAH。总之，烟草多环芳烃在口腔粘膜有氧条件下大规模微生物降解的证据仍然缺乏，虽然非丰富的多环芳烃代谢物肯定存在。
Certain soil microbes resist and metabolize polycyclic aromatic hydrocarbons (PAHs). The same is true for a subset of skin microbes. In the human mouth, oral microbes have the potential to oxidize tobacco PAHs, thereby increasing these chemicals’ ability to cause cancer of adjacent epithelium. We hypothesized that we could identify, in smokers, the oral mucosal microbes that can metabolize PAH. We isolated bacteria and fungi that survived long-term in minimal media with PAHs as the sole carbon source, under aerobic conditions, from the oral mucosa in 17 of 26 smokers and two of 14 nonsmokers. Of bacteria genera that survived harsh PAH exposure in vitro, most were found at trace levels, except for Staphylococcus, Actinomyces, and Kingella, which were more abundant. Two PAH-resistant strains of Candida albicans (C. albicans) were isolated from smokers. C. albicans was a prime candidate to contribute to carcinogenesis in tobacco users as it is found orally at high levels in tobacco users on the mucosa, and some Candida species can metabolize PAHs. However, when C. albicans isolates were tested for metabolism of two model PAH substrates, pyrene and phenanthrene, they were not capable, suggesting they cannot metabolize PAH under the conditions used. In conclusion, evidence for large scale microbial degradation of tobacco PAHs under aerobic conditions on the oral mucosa remains lacking, though nonabundant PAH metabolizers are certainly present.
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影响因子：
4.5
作者：
Al-Hebshi NN;Nasher AT;Idris AM;Chen T
通讯作者：
Chen T
影响因子：
5.2
作者：
Moussa HA;Wasfi R;Abdeltawab NF;Megahed SA
通讯作者：
Megahed SA
影响因子：
5.8
作者：
Flores-Ramirez, Rogelio;Ortega-Romero, Manolo;Diaz de Leon-Martinez, Lorena
通讯作者：
Diaz de Leon-Martinez, Lorena
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
5
作者：
Deng, Yanqin;Zhang, Yu;Yang, Min
通讯作者：
Yang, Min