High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers.
High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers.
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DOI：
10.3389/fmicb.2021.661509
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发表时间：
2021
影响因子：
5.2
通讯作者：
Megahed SA
中科院分区：
文献类型：
--
作者：
Moussa HA;Wasfi R;Abdeltawab NF;Megahed SA
口腔微生物群的组成和代谢功能受到许多因素的影响，包括吸烟，导致多种健康问题。吸烟与口腔微生物群组成和功能的变化有关。然而，目前尚不清楚某些细菌属和种的消耗是由于香烟烟雾中的特定毒素，还是间接由于与吸烟富集的细菌竞争定植。因此，本研究的目的是确定吸烟对吸烟者口腔微生物群中的微生物患病率和多环芳烃（PAHs）生物降解的影响，与非吸烟者相比，吸烟者口腔微生物群中的富集和耗尽口腔细菌。收集来自吸烟者和非吸烟者的口腔冲洗液样品（n = 23，12名吸烟者和11名非吸烟者），并筛选口腔细菌菌株变形链球菌、乳杆菌属，和韦荣球菌属（Veillonella spp.）比较计数，S。与非吸烟者相比，吸烟者中的变形链球菌、土别弧菌和舞毒菌的数量更高，而乳杆菌则更高。在非吸烟者中更高。吸烟者中以发酵乳杆菌为主，占总乳杆菌的91.67%。分离株以蒽为唯一碳源，以2，6-二氯酚靛酚（2，6-DCPIP）为指示剂，研究了单菌和混合菌培养条件下蒽的生物降解潜力。S.与从非吸烟者中回收的那些相比，从吸烟者中回收的变异株显示出更高的蒽降解。非吸烟者中的小弧菌对蒽的厌氧降解活性最高。研究了乳杆菌对蒽的生物降解潜力。是可变的。共培养物中分离细菌的组合表明，乳杆菌属。沿着S.变形杆菌和韦荣球菌属（Veillonella spp.）总之，观察到吸烟引起的口腔生态失调不仅是由于口腔细菌相对丰度的变化，而且还扩展到细菌功能，如本研究中测试的蒽生物降解。微生物-微生物相互作用改变了蒽的生物降解潜力和微生物混合物的生长相比，其相应的单一菌株，这些变化根据构成细菌不同。
The composition and metabolic functions of oral microbiota are affected by many factors including smoking leading to several health problems. Cigarette smoking is associated with changes in oral microbiota composition and function. However, it is not known if the depletion of certain bacterial genera and species is due to specific toxins in cigarette smoke, or indirectly due to competition for colonization with smoking-enriched bacteria. Therefore, the aim of this study was to determine the effect of cigarette smoking on the microbial prevalence and polycyclic aromatic hydrocarbons (PAHs) biodegradation of selected enriched and depleted oral bacteria from oral microbiota of smokers compared to that in non-smokers. Samples of oral rinse from smokers and non-smokers were collected (n = 23, 12 smokers and 11 non-smokers) and screened for oral bacterial strains of Streptococcus mutans, Lactobacillus spp., and Veillonella spp. Comparing counts, S. mutans, V. tobetsuensis, and V. dispar showed higher counts in smokers compared to non-smokers while the Lactobacillus spp. were higher in non-smokers. Lactobacillus fermentum was prevalent in smokers, representing 91.67% of the total Lactobacillus spp. isolates. The biodegradation potential of anthracene; a representative of PAHs of collected isolates, in single and mixed cultures, was assayed with anthracene as the sole source of carbon using 2,6-dichlorophenol indophenol (2,6-DCPIP) as indicator. S. mutans isolates recovered from smokers showed higher degradation of anthracene compared to those recovered from non-smokers. The anaerobic anthracene biodegradation activity of V. parvula isolates from non-smokers was the highest among all isolates of the three recovered genera from the same subject. The anthracene biodegradation potential of Lactobacillus spp. was variable. Combinations of isolated bacteria in co-cultures showed that Lactobacillus spp. interfered with anthracene biodegradation ability along with the viable counts of S. mutans and Veillonella spp. In conclusion, oral dysbiosis due to cigarette smoking was observed not only due to changes in oral bacterial relative abundance but also extended to bacterial functions such as anthracene biodegradation tested in this study. Microbe–microbe interactions changed the anthracene biodegradation potential and growth of the microbial mixture compared to their corresponding single isolates, and these changes differ according to the constituting bacteria.
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影响因子：
5.7
作者：
Do T;Sheehy EC;Mulli T;Hughes F;Beighton D
通讯作者：
Beighton D
影响因子：
3.7
作者：
Abranches J;Zeng L;Kajfasz JK;Palmer SR;Chakraborty B;Wen ZT;Richards VP;Brady LJ;Lemos JA
通讯作者：
Lemos JA
影响因子：
3.8
作者：
de Souza Rodrigues, Jeisa Zielle;Passos, Manuela Ribeiro;Yatsuda, Regiane
通讯作者：
Yatsuda, Regiane
影响因子：
2.5
作者：
Chowdhury, Rocky;Dhar, Kartik;Uddin, Mohammad
通讯作者：
Uddin, Mohammad
影响因子：
6.7
作者：
Bizzarro, Sergio;Loos, Bruno G.;Zaura, Egija
通讯作者：
Zaura, Egija