Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis.
Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis.
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DOI：
10.1186/s13071-021-04713-6
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发表时间：
2021-04-20
影响因子：
3.2
通讯作者：
Zhou DH
中科院分区：
文献类型：
--
作者：
Cui LL;Zhou CX;Han B;Wang SS;Li SY;Xie SC;Zhou DH
刚地弓形虫是一种引起弓形虫病的专性细胞内寄生虫。尿液是一种容易获得的临床样本，已广泛应用于诊断目的。然而，尿蛋白质组在T。从未对弓形虫感染进行过调查。从急性感染[感染后11天（DPI）]的BALB/c小鼠、慢性感染（35 DPI）小鼠和健康对照小鼠中获得24小时尿样，并使用无标记液相色谱串联质谱分析进行分析。我们在1802个蛋白质上鉴定了总共13，414个肽，其中169个和47个蛋白质分别在急性和慢性感染阶段显著差异表达。聚类分析显示各组间蛋白质组差异明显。基因本体分析表明，在急性感染中检测到的大量差异表达蛋白（DEPs）与生物结合活性和单个生物过程相关。KEGG途径富集分析表明，这些DEP中的大多数参与疾病相关和代谢途径。我们的研究结果揭示了T.本研究获得的数据将增强我们对弓形虫感染的宿主反应的理解，并导致新的诊断生物标志物的鉴定。在线版本包含补充材料，可通过10.1186/s13071-021-04713-6获得。
Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis. Urine is an easily obtained clinical sample that has been widely applied for diagnostic purposes. However, changes in the urinary proteome during T. gondii infection have never been investigated. Twenty four-hour urine samples were obtained from BALB/c mice with acute infection [11 days post infection (DPI)], mice with chronic infection (35 DPI) and healthy controls, and were analyzed using a label-free liquid chromatography tandem mass spectrometry analysis. We identified a total of 13,414 peptides on 1802 proteins, of which 169 and 47 proteins were significantly differentially expressed at acute and chronic infection phases, respectively. Clustering analysis revealed obvious differences in proteome profiles among all groups. Gene ontology analysis showed that a large number of differentially expressed proteins (DEPs) detected in acute infection were associated with biological binding activity and single-organism processes. KEGG pathway enrichment analysis showed that the majority of these DEPs were involved in disease-related and metabolic pathways. Our findings revealed global reprogramming of the urine proteome following T. gondii infection, and data obtained in this study will enhance our understanding of the host responses to T. gondii infection and lead to the identification of new diagnostic biomarkers. The online version contains supplementary material available at 10.1186/s13071-021-04713-6.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
14.9
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Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
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慢性弓形虫感染诱导的野生型和CD44-小鼠脑蛋白质组差异
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2
作者：
Yang, Jing;Du, Fen;Zhao, Junlong
通讯作者：
Zhao, Junlong
影响因子：
2
作者：
Li, Qing-Run;Fan, Ke-Xin;Zeng, Rong
通讯作者：
Zeng, Rong
影响因子：
3.4
作者：
Thongboonkerd, V;Malasit, P
通讯作者：
Malasit, P