Molecular characterization of fructose-1,6-bisphosphatase 1b in blunt snout bream Megalobrama amblycephala and the transcriptional response to glucose loading after the adaptation to high-carbohydrate diets
Molecular characterization of fructose-1,6-bisphosphatase 1b in blunt snout bream Megalobrama amblycephala and the transcriptional response to glucose loading after the adaptation to high-carbohydrate diets
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团头鲂果糖 1,6-双磷酸酶 1b 的分子特征以及适应高碳水化合物饮食后对葡萄糖负荷的转录反应
DOI：
10.1007/s10695-017-0376-x
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发表时间：
2017-05
影响因子：
2.9
通讯作者：
Liu Wen-Bin
中科院分区：
文献类型：
--
作者：
Li Xiang-Fei;Xu Chao;Jiang Guang-Zhen;Zhang Ding-Dong;Liu Wen-Bin
本研究旨在表征团头鲂果糖-1,6-双磷酸酶 1b (FBP1b) 的全长互补 DNA (cDNA)，并研究其在适应高碳水化合物饮食后对葡萄糖施用的转录反应。获得的cDNA全长1435bp，开放阅读框1014bp。序列比对和系统发育分析显示，大多数鱼类和其他脊椎动物具有高度保守性（76-96%），保留了 1 个 N 连接糖基化位点、1 个 N 末端乙酰化位点、13 个磷酸化位点、1 个果糖 1,6-二磷酸酶 (FBPase) 活性位点、5 个金属结合位点、4 个底物结合位点和几个 AMP 结合位点。 FBP1b 的信使 RNA (mRNA) 水平在肝脏中观察到最高，其次是肠道，而在心脏、鳃和眼睛中检测到的值相对较低。然后，在喂食两种膳食碳水化合物水平（30％和42％）11周后，腹腔注射每公斤体重1.67克葡萄糖的鱼的肝脏中FBP1b的mRNA水平和FBP酶活性均被测定。葡萄糖负荷后，两种处理的FBP1b mRNA水平在8 h时均显着下降至基础值，随后略有上升。然而，酶活性在前4 h内没有表现出统计学差异，但随着时间的进一步增加，酶活性显着增加。此外，随着膳食碳水化合物水平的增加，mRNA 水平和活性均显着下降。结果表明，团头鳅的FBP1b与其他脊椎动物的FBP1b具有较高的相似性。给予葡萄糖后，其在肝脏中的 mRNA 表达显着下调，长期适应富含碳水化合物的饮食后也是如此。
This study aimed to characterize the full-length complementary DNA (cDNA) of fructose-1,6-bisphosphatase 1b (FBP1b) from fish Megalobrama amblycephala, and investigate its transcriptional response to glucose administration after the adaptation to high-carbohydrate diets. The cDNA obtained covered 1435 bp with an open reading frame of 1014 bp. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (76-96%) among most fish and other vertebrates, retaining one N-linked glycosylation site, one N-terminal acetylation site, 13 phosphorylation sites, one fructose-1,6-bisphosphatase (FBPase) active site, five metal-binding sites, four substrate-binding sites, and several AMP-binding sites. The highest messenger RNA (mRNA) level of FBP1b was observed in liver followed by intestine, whereas relatively low values were detected in heart, gill, and eye. Then, the mRNA levels of FBP1b and the FBPase activity were both determined in the liver of fish injected intraperitoneally with 1.67 g glucose per kilogram body weight after being fed two dietary carbohydrate levels (30 and 42%) for 11 weeks. After the glucose load, the mRNA levels of FBP1b in both treatments decreased significantly to the basal value at 8 h and showed a slight increase afterward. However, the enzymatic activity showed no statistical difference during the first 4 h, but increased remarkably with further increasing times. In addition, both the mRNA levels and activities decreased significantly with increasing dietary carbohydrate levels. The results indicated that the FBP1b of M. amblycephala shared a high similarity with that of the other vertebrates. Its mRNA expression in liver was downregulated remarkably by a glucose administration, as also held true after the long-term adaptation of a carbohydrate-rich diet.
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影响因子：
8
作者：
Iynedjian, P. B.
通讯作者：
Iynedjian, P. B.
DOI：
10.1073/pnas.89.9.3746
发表时间：
1992-05
影响因子：
11.1
作者：
J. L. Rosa;A. Tauler;A. Lange;S. Pilkis;R. Bartrons
通讯作者：
J. L. Rosa;A. Tauler;A. Lange;S. Pilkis;R. Bartrons
影响因子：
3.5
作者：
Arthur H. Smith
通讯作者：
Arthur H. Smith
DOI：
--
发表时间：
2003
期刊：
--
影响因子：
--
作者：
C. Dieffenbach;G. Dveksler
通讯作者：
C. Dieffenbach;G. Dveksler
DOI：
--
发表时间：
2001-07
期刊：
The Journal of experimental biology
影响因子：
--
作者：
S. Panserat;É. Plagnes-Juan;S. Kaushik
通讯作者：
S. Panserat;É. Plagnes-Juan;S. Kaushik