Comparison of the expression of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α in oral squamous cell carcinoma and lymph node metastases of humans and mice.
Comparison of the expression of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α in oral squamous cell carcinoma and lymph node metastases of humans and mice.
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人和小鼠口腔鳞状细胞癌及淋巴结转移中TGFαβ1、Eαcadherin、Nαcadherin、TP53、RB1CC1和HIFα1α的表达比较
DOI：
10.3892/ol.2017.7456
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发表时间：
2018-03
期刊：
影响因子：
2.9
通讯作者：
Liang F
中科院分区：
文献类型：
--
作者：
Guo M;Mu Y;Yu D;Li J;Chen F;Wei B;Bi S;Yu J;Liang F
本研究的目的是通过比较人类和小鼠口腔鳞状细胞癌（OSCC）不同阶段转化生长因子（TGF）-β1、E-钙粘蛋白、N-钙粘蛋白、肿瘤蛋白（TP）53、RB1诱导卷曲螺旋（RB1CC）1和缺氧诱导因子（HIF）-1α的表达水平，证明小鼠模型密切模拟人类口腔癌的进展。采用免疫组织化学染色法检测正常口腔黏膜、口腔黏膜不典型增生、口腔鳞状细胞癌原发灶及淋巴结转移癌组织中TGF-β1、E-cadherin、N-cadherin、TP53、RB1CC1、HIF-1α的表达水平。组织样本取自人类标本和由饮用水中的 4-硝基喹啉-1-氧化物诱导的口腔癌淋巴转移的 Balb/c 小鼠模型。结果显示，在口腔鳞状细胞癌的各个阶段，人和小鼠之间TGF-β1、E-cadherin、N-cadherin、TP53、RB1CC1和HIF-1α的表达水平均无显着差异（P>0.05）。在人和小鼠的 OSCC 的不同阶段，TGF-β1、N-cadherin、TP53 和 RB1CC1 的表达均增加。在人和小鼠样本中，从正常口腔粘膜到口腔癌，E-钙粘蛋白的表达减少，而在淋巴结转移中增加。在人和小鼠样本中，从正常口腔粘膜到口腔癌，HIF-1α的表达增加，而在淋巴结转移中减少。此外，人和小鼠样本中p53的表达与RB1CC1的表达呈正相关（r=0.971，P=0.029；r=0.97，P=0.03）。总体而言，多种分子在人和小鼠癌症中的相似表达证明本研究建立的口腔癌淋巴转移的小鼠模型可能非常模仿人类口腔癌。
The aim of the present study was to prove that a mouse model closely simulates human oral cancer progression by comparing the expression levels of transforming growth factor (TGF)-β1, E-cadherin, N-cadherin, tumor protein (TP)53, RB1 inducible coiled-coil (RB1CC)1 and hypoxia inducible factor (HIF)-1α at different stages of oral squamous cell carcinoma (OSCC) in humans and mice. The expression levels of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1, and HIF-1α were detected by immunohistochemical staining in normal oral mucosa, oral mucosa dysplasia, OSCC primary tumor and carcinoma tissues from lymph node metastases. Tissue samples were obtained from human specimens and the Balb/c mouse model of lymphatic metastases oral carcinoma, induced by 4-nitroquinoline-1-oxide in drinking water. The results indicated no significant differences in the expression levels of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α between humans and mice, at any stage of OSCC examined (P>0.05). The expression of TGF-β1, N-cadherin, TP53 and RB1CC1 increased in different stages of OSCC in both humans and mice. The expression of E-cadherin decreased from normal oral mucosa to OSCC, and increased in lymph node metastases in both human and mouse samples. The expression of HIF-1α increased from normal oral mucosa to OSCC, and decreased in lymph node metastases in both human and mouse samples. Additionally, the expression of p53 was positively correlated with that of RB1CC1 in human and mouse samples (r=0.971, P=0.029; r=0.97, P=0.03). Overall, the similar expression of multiple molecules in both human and mouse carcinoma prove that the mouse model of lymphatic metastases from oral carcinoma established in the present study may closely mimic human oral cancer.
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影响因子：
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