Response of lymphocyte subsets and cytokines to Shenyang prescription in Sprague-Dawley rats with tongue squamous cell carcinomas induced by 4NQO.
Response of lymphocyte subsets and cytokines to Shenyang prescription in Sprague-Dawley rats with tongue squamous cell carcinomas induced by 4NQO.
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DOI：
10.1186/1471-2407-7-40
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发表时间：
2007-03-05
期刊：
影响因子：
3.8
通讯作者：
Chen, Wantao
中科院分区：
文献类型：
--
作者：
Jiang, Canhua;Ye, Dongxia;Qiu, Weiliu;Zhang, Xiuli;Zhang, Zhiyuan;He, Di;Zhang, Ping;Chen, Wantao
本研究旨在探讨大鼠免疫功能与肿瘤进展的关系，以及中药“沈阳”方对肿瘤免疫功能的影响。通过饮用水向80只SD大鼠给予4-硝基喹啉-1-氧化物（4 NQO）长达36周。本文报道61只大鼠舌鳞状细胞癌的病理学检查结果。对各组大鼠分别给予沈阳方治疗，并于治疗前后取血。流式细胞术测定淋巴细胞亚群。血清Th 1和Th 2型细胞因子的酶联免疫吸附试验进行了评估。随着舌根癌的进展，舌根癌患者外周血中CD 3 + CD 4 + T淋巴细胞、NK细胞及IFN-γ、IL-2水平逐渐下降，而CD 3 + CD 8 + T淋巴细胞及IL-4、IL-10水平逐渐升高。CD 4 +/CD 8+比值在癌症组中低于对照组。服用“沈阳”方后，CD 3 + CD 4 + T淋巴细胞、NK细胞、IFN-γ和IL-2水平升高，而CD 3 + CD 8 + T淋巴细胞计数和IL-4、IL-10水平降低。4 NQO诱发的口腔黏膜损伤是研究口腔黏膜癌变的良好模型。4 NQO诱导的SCC大鼠表现出淋巴细胞亚群的异常和从Th 1型向Th 2型的转变，这是评估抗癌剂对免疫的影响的良好模型。口腔癌的进展与免疫功能的侵袭性紊乱有关。沈阳方具有改善免疫功能紊乱的作用。
The study was designed to investigate immunocompetence in relation to cancer progression in rat and to assess the effect of the traditional Chinese anti-cancer medicine, "Shenyang" prescription, on immunity. 4-Nitroquinoline-1-oxide (4NQO) was administered to 80 Sprague-Dawley (SD) rats via the drinking water for up to 36 weeks. Tongue squamous cell carcinoma (SCC) was confirmed by pathological examination in 61 rats. "Shenyang" prescription was administered to subgroups of these rats, and blood samples were taken before and after treatment. Lymphocyte subsets were determined by flow cytometry. Serum Th1 and Th2-type cytokines were assessed by an enzyme-linked immunosorbent assay. As the cancer progressed at the tongue root, the percentage of CD3+CD4+ T lymphocytes and NK cells and the levels of IFN-γ and IL-2 decreased gradually, while the percentage of CD3+CD8+ T lymphocytes and the levels of IL-4 and IL-10 increased. The CD4+/CD8+ ratios were lower in the cancer groups than in the control group. However, after administering "Shenyang" prescription, the levels of CD3+CD4+ T lymphocytes, NK cells, IFN-γ and IL-2 increased, while the CD3+CD8+ T lymphocyte counts and the levels of IL-4 and IL-10 decreased. 4NQO-induced lesions were good models for exploring oral cavity carcinogenesis. The rats with 4NQO-induced SCC demonstrated abnormalities in lymphocyte subsets and a shift from Th1-type to Th2-type, which were good models for assessing the effect of anticancer agent on immunity. Oral cancer progression was associated with an aggressive disturbance of immune function. "Shenyang" prescription has the ability to improve the disturbance of immune function.
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DOI：
10.1084/jem.188.12.2357
发表时间：
1998-12-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hung K;Hayashi R;Lafond-Walker A;Lowenstein C;Pardoll D;Levitsky H
通讯作者：
Levitsky H
DOI：
10.1002/hed.2880160506
发表时间：
1994-09-01
影响因子：
2.9
作者：
HAWKINS, BL;HENIFORD, BW;HENDLER, FJ
通讯作者：
HENDLER, FJ
影响因子：
3.8
作者：
Kuninaka, S;Yano, T;Ichinose, Y
通讯作者：
Ichinose, Y
影响因子：
11.5
作者：
Kuss, I;Hathaway, B;Whiteside, TL
通讯作者：
Whiteside, TL
影响因子：
3.8
作者：
Lathers, DMR;Young, MRI
通讯作者：
Young, MRI