Mapping mesoscale cortical connectivity in monkey sensorimotor cortex with optical imaging and microstimulation.
Mapping mesoscale cortical connectivity in monkey sensorimotor cortex with optical imaging and microstimulation.
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DOI：
10.1002/cne.24918
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Roe AW
中科院分区：
文献类型：
--
作者：
Friedman RM;Morone KA;Gharbawie OA;Roe AW
为了在中尺度上绘制体内皮层电路，我们采用了一种新的方法来绘制区域间功能连接。皮层内电微刺激（ICMS）结合内在信号的光学成像（OIS）被用来映射麻醉松鼠猴体感皮层区的功能连接。ICMS产生的激活是局灶性的，并显示刺激强度依赖性的反应。在Brodmann区域3b、1、2、3a和M1的颗粒上层中的ICMS诱发区域间激活模式，这些模式在地形上是适当的，并且与已知的解剖连接性一致。具体而言，ICMS揭示了区域3b与区域1的连接;区域1与区域2和3a的连接;区域2与区域1，3a和M1的连接;区域3a与区域M1，1和2的连接;以及M1与区域3a，1和2的连接。这些体感连接模式让人想起解剖学上观察到的前馈模式，虽然反馈的贡献也可能存在。与解剖学连接性进一步一致，区域内和区域内激活模式为斑块状，斑块大小为200-300 μm。总之，ICMS与OIS是一种新的方法，用于映射区域间和区域内的连接在体内。前馈和反馈解剖连接的比较进行了讨论。通过将皮质内电微刺激（ICMS）与内在光学成像相结合，我们发现猴皮质（区域3a，3b，1，2和M1）中单个手指位置的局灶性ICMS揭示了已知的解剖学连接。这种研究皮层连接的新方法是在体内，快速，有针对性的，中尺度的。
To map in vivo cortical circuitry at the mesoscale we applied a novel approach to map inter-areal functional connectivity. Electrical intracortical microstimulation (ICMS) in conjunction with optical imaging of intrinsic signals (OIS) was used map functional connections in somatosensory cortical areas in anesthetized squirrel monkeys. ICMS produced activations that were focal and that displayed responses which were stimulation intensity dependent. ICMS in supragranular layers of Brodmann areas 3b, 1, 2, 3a, and M1 evoked inter-areal activation patterns that were topographically appropriate and appeared consistent with known anatomical connectivity. Specifically, ICMS revealed area 3b connections with area 1; area 1 connections with areas 2 and 3a; area 2 connections with areas 1, 3a, and M1; area 3a connections with areas M1, 1, and 2; and M1 connections with areas 3a, 1, and 2. These somatosensory connectivity patterns were reminiscent of feedforward patterns observed anatomically, although feedback contributions are also likely present. Further consistent with anatomical connectivity, intra-areal and intra-areal patterns of activation were patchy with patch sizes of 200–300 μm. In summary, ICMS with OIS is a novel approach for mapping inter-areal and intra-areal connections in vivo. Comparisons with feedforward and feedback anatomical connectivity is discussed. By combining intracortical electrical microstimulation (ICMS) with intrinsic optical imaging, we find that focal ICMS at single digit locations in monkey cortex (areas 3a, 3b, 1, 2, and M1) reveals known anatomical connections. This novel method of studying cortical connections is in vivo, rapid, targeted, and mesoscale.
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影响因子：
3.1
作者：
Pálfi E;Zalányi L;Ashaber M;Palmer C;Kántor O;Roe AW;Friedman RM;Négyessy L
通讯作者：
Négyessy L
影响因子：
64.8
作者：
SALZMAN, CD;BRITTEN, KH;NEWSOME, WT
通讯作者：
NEWSOME, WT
影响因子：
2.5
作者：
Friedman, Robert M.;Chen, Li Min;Roe, Anna W.
通讯作者：
Roe, Anna W.
影响因子：
2.5
作者：
JONES, EG;COULTER, JD;HENDRY, SHC
通讯作者：
HENDRY, SHC
影响因子：
2.5
作者：
Chen, LM;Friedman, RM;Roe, AW
通讯作者：
Roe, AW