Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors
Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors
复制标题
酵母信息素受体内吞作用所需的顺式和反式作用功能
作者：
N. Davis;J. Horecka;G. Sprague
酿酒酵母 a 因子受体 (STE3) 受到两种胞吞模式的影响：在没有配体的情况下发生的组成过程和由配体结合触发的调节过程。这两个过程都会导致受体递送至液泡进行降解。删除部分 COOH 末端胞质结构域的受体突变体无法进行组成型内化，但不能进行配体依赖性内化。损害组成型内吞作用的反式作用突变体已被分离出来。其中之一，ren1-1，在内吞途径的后期被阻断，因为受体在液泡前内体样区室中积累。 REN1 与 VPS2 相同，VPS2 是将新合成的液泡酶递送到液泡所需的基因。基于这一特性，我们提出了一个模型，其中液泡的运输途径——内吞途径和液泡生物发生途径——在中间内吞室处合并。由于受体也在 ren1 细胞表面积累，受体可能从假定的内体再循环到表面，或者 REN1 也可能需要执行内吞作用的早期步骤。
The Saccharomyces cerevisiae a-factor receptor (STE3) is subject to two modes of endocytosis: a constitutive process that occurs in the absence of ligand and a regulated process that is triggered by binding of ligand. Both processes result in delivery of the receptor to the vacuole for degradation. Receptor mutants deleted for part of the COOH- terminal cytoplasmic domain are disabled for constitutive, but not ligand-dependent internalization. Trans-acting mutants that impair constitutive endocytosis have been isolated. One of these, ren1-1, is blocked at a late step in the endocytic pathway, as receptor accumulates in a prevacuolar endosome-like compartment. REN1 is identical to VPS2, a gene required for delivery of newly synthesized vacuolar enzymes to the vacuole. Based on this identity, we suggest a model in which the transport pathways to the vacuole--the endocytic pathway and the vacuolar biogenesis pathway--merge at an intermediate endocytic compartment. As receptor also accumulates at the surface of ren1 cells, receptor may recycle from the putative endosome to the surface, or REN1 may also be required to carry out an early step in endocytosis.
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影响因子：
2.9
作者：
J. Schlessinger
通讯作者：
J. Schlessinger
影响因子：
11.4
作者：
KUCHLER, K;STERNE, RE;THORNER, J
通讯作者：
THORNER, J
影响因子：
56.9
作者：
LEMMON, SK;JONES, EW
通讯作者：
JONES, EW
酵母细胞融合蛋白 FUS1 被 O-糖基化并跨越质膜。
DOI：
10.1073/pnas.86.24.9916
发表时间：
1989
影响因子：
11.1
作者：
Trueheart,J;Fink,GR
通讯作者：
Fink,GR
内吞作用和内化信号。
影响因子：
7.5
作者：
Trowbridge,IS
通讯作者：
Trowbridge,IS