Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.
Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.
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DOI：
10.1083/jcb.201706097
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发表时间：
2018-03-05
期刊：
影响因子：
--
通讯作者：
Burridge K
中科院分区：
文献类型：
--
作者：
Graham DM;Andersen T;Sharek L;Uzer G;Rothenberg K;Hoffman BD;Rubin J;Balland M;Bear JE;Burridge K
The nucleus plays critical physical roles during cell polarization, migration, and mechanotransduction. Graham et al. explore these processes in its absence. Although polarization and migration occur, cells lacking a nucleus revealed an altered mechanoresponse that is consistent with the nucleus regulating cell contractility. The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical necessity of the nucleus during cell polarization and directed migration. Using enucleated mammalian cells (cytoplasts), we found that polarity establishment and cell migration in one dimension (1D) and two dimensions (2D) occur without the nucleus. Cytoplasts directionally migrate toward soluble (chemotaxis) and surface-bound (haptotaxis) extracellular cues and migrate collectively in scratch-wound assays. Consistent with previous studies, migration in 3D environments was dependent on the nucleus. In part, this likely reflects the decreased force exerted by cytoplasts on mechanically compliant substrates. This response is mimicked both in cells with nucleocytoskeletal defects and upon inhibition of actomyosin-based contractility. Together, our observations reveal that the nucleus is dispensable for polarization and migration in 1D and 2D but critical for proper cell mechanical responses.
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影响因子：
21.3
作者：
Case LB;Waterman CM
通讯作者：
Waterman CM
DOI：
10.1083/jcb.116.5.1157
发表时间：
1992-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Euteneuer U;Schliwa M
通讯作者：
Schliwa M
DOI：
10.1083/jcb.201004118
发表时间：
2010-10-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fridolfsson HN;Starr DA
通讯作者：
Starr DA
DOI：
10.1038/nrm3112
发表时间：
2011-05
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16.6
作者：
Bangasser BL;Shamsan GA;Chan CE;Opoku KN;Tüzel E;Schlichtmann BW;Kasim JA;Fuller BJ;McCullough BR;Rosenfeld SS;Odde DJ
通讯作者：
Odde DJ