Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei.
Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei.
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DOI：
10.1083/jcb.201004118
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发表时间：
2010-10-04
期刊：
影响因子：
--
通讯作者：
Starr DA
中科院分区：
文献类型：
--
作者：
Fridolfsson HN;Starr DA
分子马达kinesin-1 dynein在细胞质障碍周围导航迁移的细胞核。Kinesin-1和dynein被秀丽线虫klarsicht/ANC-1/Syne同源(Kash)蛋白UNC-83招募到核膜上，以移动细胞核。这些马达如何协调以调节核迁移的机制尚不清楚。采用延时差示干涉造影法和荧光成像技术研究了胚胎皮下核迁移的动力学过程，并确定了微管的动力学和极性。野生型细胞核在迁移过程中表现出双向移动，也能够滚动通过细胞质颗粒。UNC-83、UNC-84和Kinesin-1突变体存在严重的核迁移缺陷。在没有dynein的情况下，核迁移正常启动，但缺乏双向移动，并显示出核滚动的缺陷，这意味着dynein在解决细胞质障碍方面存在问题。在核迁移过程中，微管是高度动态的。EB1：：绿色荧光蛋白成像显示微管朝着核迁移方向极化。这种微管的组织结构符合我们的模型，即kinesin-1使细胞核向前移动，而dynein则将细胞核向后移动一小段时间，以绕过细胞障碍。
The molecular motors kinesin-1 dynein navigate migrating nuclei around cytoplasmic roadblocks. Kinesin-1 and dynein are recruited to the nuclear envelope by the Caenorhabditis elegans klarsicht/ANC-1/Syne homology (KASH) protein UNC-83 to move nuclei. The mechanisms of how these motors are coordinated to mediate nuclear migration are unknown. Time-lapse differential interference contrast and fluorescence imaging of embryonic hypodermal nuclear migration events were used to characterize the kinetics of nuclear migration and determine microtubule dynamics and polarity. Wild-type nuclei display bidirectional movements during migration and are also able to roll past cytoplasmic granules. unc-83, unc-84, and kinesin-1 mutants have severe nuclear migration defects. Without dynein, nuclear migration initiates normally but lacks bidirectional movement and shows defects in nuclear rolling, implicating dynein in resolution of cytoplasmic roadblocks. Microtubules are highly dynamic during nuclear migration. EB1::green fluorescence protein imaging demonstrates that microtubules are polarized in the direction of nuclear migration. This organization of microtubules fits with our model that kinesin-1 moves nuclei forward and dynein functions to move nuclei backward for short stretches to bypass cellular roadblocks.
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影响因子：
4
作者：
Levy JR;Holzbaur EL
通讯作者：
Holzbaur EL
DOI：
10.1083/jcb.124.4.491
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hresko MC;Williams BD;Waterston RH
通讯作者：
Waterston RH
DOI：
10.1083/jcb.200908075
发表时间：
2009-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ally S;Larson AG;Barlan K;Rice SE;Gelfand VI
通讯作者：
Gelfand VI
影响因子：
64.5
作者：
Chikashige, Y;Tsutsumi, C;Hiraoka, Y
通讯作者：
Hiraoka, Y
影响因子：
2.7
作者：
Leung, B;Hermann, GJ;Priess, JR
通讯作者：
Priess, JR