Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.
Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.
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DOI：
10.1093/jxb/ert055
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Fotopoulos V
中科院分区：
文献类型：
--
作者：
Christou A;Manganaris GA;Papadopoulos I;Fotopoulos V
最近发现硫化氢（H2S）作为一种有效的引发剂。以草莓（Fragaria × ananassa cv.用H2S供体硫氢化钠（NaHS; 100 μM，48 h）处理Camarosa）根，可诱导持久的引发效应，并对随后的100 mM NaCl或10%（w/v）PEG-6000处理7 d产生耐受。H2S预处理的根导致增加叶片叶绿素荧光，气孔导度和叶片相对含水量，以及较低的脂质过氧化水平相比，植物直接受到盐和非离子渗透胁迫，从而表明H2S预处理的非生物胁迫因素引起的细胞损伤的系统缓解作用。此外，根预处理与NaHS导致在草莓植物中的氧化和亚硝化胁迫的最小化，表现为通过较低水平的NO和H2 O2的合成在叶片和高抗坏血酸和谷胱甘肽氧化还原状态的维护，随后的盐和非离子渗透胁迫。对关键抗氧化剂（cAPX、CAT、MnSOD、GR）、抗坏血酸和谷胱甘肽生物合成（GCS、GDH、GS）、转录因子（DREB）和盐过度敏感（SOS）途径（SOS 2-like、SOS 3-like、SOS 4）基因的定量实时RT-PCR基因表达分析表明，H2S在多个转录途径的协调调节中起关键作用。H2S的改善效果更明显，在草莓植物进行两种胁迫条件下，NaHS根预处理后立即，而不是在植物进行胁迫条件下3 d后根预处理。总的来说，H2S预处理的植物设法克服盐和非离子渗透胁迫的有害影响，通过控制氧化和亚硝化细胞损伤，通过提高性能的抗氧化机制和SOS途径的协调调节，从而提出了一种新的作用H2S在植物引发，特别是在水果作物，如草莓。
Hydrogen sulfide (H2S) has been recently found to act as a potent priming agent. This study explored the hypothesis that hydroponic pretreatment of strawberry (Fragaria × ananassa cv. Camarosa) roots with a H2S donor, sodium hydrosulfide (NaHS; 100 μM for 48h), could induce long-lasting priming effects and tolerance to subsequent exposure to 100mM NaCI or 10% (w/v) PEG-6000 for 7 d. Hydrogen sulfide pretreatment of roots resulted in increased leaf chlorophyll fluorescence, stomatal conductance and leaf relative water content as well as lower lipid peroxidation levels in comparison with plants directly subjected to salt and non-ionic osmotic stress, thus suggesting a systemic mitigating effect of H2S pretreatment to cellular damage derived from abiotic stress factors. In addition, root pretreatment with NaHS resulted in the minimization of oxidative and nitrosative stress in strawberry plants, manifested via lower levels of synthesis of NO and H2O2 in leaves and the maintenance of high ascorbate and glutathione redox states, following subsequent salt and non-ionic osmotic stresses. Quantitative real-time RT-PCR gene expression analysis of key antioxidant (cAPX, CAT, MnSOD, GR), ascorbate and glutathione biosynthesis (GCS, GDH, GS), transcription factor (DREB), and salt overly sensitive (SOS) pathway (SOS2-like, SOS3-like, SOS4) genes suggests that H2S plays a pivotal role in the coordinated regulation of multiple transcriptional pathways. The ameliorative effects of H2S were more pronounced in strawberry plants subjected to both stress conditions immediately after NaHS root pretreatment, rather than in plants subjected to stress conditions 3 d after root pretreatment. Overall, H2S-pretreated plants managed to overcome the deleterious effects of salt and non-ionic osmotic stress by controlling oxidative and nitrosative cellular damage through increased performance of antioxidant mechanisms and the coordinated regulation of the SOS pathway, thus proposing a novel role for H2S in plant priming, and in particular in a fruit crop such as strawberry.
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