Proteomic analysis of circulating extracellular vesicles identifies potential markers of breast cancer progression, recurrence, and response.
Proteomic analysis of circulating extracellular vesicles identifies potential markers of breast cancer progression, recurrence, and response.
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DOI：
10.1126/sciadv.aba5714
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发表时间：
2020-10
期刊：
影响因子：
13.6
通讯作者：
Lev S
中科院分区：
文献类型：
--
作者：
Vinik Y;Ortega FG;Mills GB;Lu Y;Jurkowicz M;Halperin S;Aharoni M;Gutman M;Lev S
细胞外小囊泡的蛋白质组学分析可将乳腺癌患者与健康女性区分开来。循环小细胞外囊泡（sev）的蛋白质组学分析为乳腺癌（BC）的早期检测和治疗监测提供了一种有前途的、无创的方法。我们描述了一种相对低成本、快速、可靠的方法，从BC患者血浆中分离sev，并通过半定量蛋白质组学分析其蛋白质含量。从健康对照和BC患者手术前后不同疾病阶段的血浆中分离出sev富集组分。利用逆相蛋白阵列对富含sev的组分进行蛋白质组学分析，发现了区分BC患者与健康个体的7种蛋白的特征，其中FAK和纤维连接蛋白显示出较高的诊断准确性。sev的大小在疾病晚期显著减少，并伴有阶段特异性蛋白特征。此外，我们观察到基于蛋白质的健康对照、化疗治疗和未治疗的术后样本的不同簇，以及癌症复发高风险的预测因子，这表明应用方法值得开发用于高级诊断。
Proteomic analysis of small extracellular vesicles differentiates patients with breast cancer from healthy women. Proteomic profiling of circulating small extracellular vesicles (sEVs) represents a promising, noninvasive approach for early detection and therapeutic monitoring of breast cancer (BC). We describe a relatively low-cost, fast, and reliable method to isolate sEVs from plasma of BC patients and analyze their protein content by semiquantitative proteomics. sEV-enriched fractions were isolated from plasma of healthy controls and BC patients at different disease stages before and after surgery. Proteomic analysis of sEV-enriched fractions using reverse phase protein array revealed a signature of seven proteins that differentiated BC patients from healthy individuals, of which FAK and fibronectin displayed high diagnostic accuracy. The size of sEVs was significantly reduced in advanced disease stage, concomitant with a stage-specific protein signature. Furthermore, we observed protein-based distinct clusters of healthy controls, chemotherapy-treated and untreated postsurgery samples, as well as a predictor of high risk of cancer relapse, suggesting that the applied methods warrant development for advanced diagnostics.
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影响因子：
5.7
作者：
Ludwik KA;Campbell JP;Li M;Li Y;Sandusky ZM;Pasic L;Sowder ME;Brenin DR;Pietenpol JA;O'Doherty GA;Lannigan DA
通讯作者：
Lannigan DA
DOI：
10.1056/nejmoa1804710
发表时间：
2018-07-12
期刊：
The New England journal of medicine
影响因子：
--
作者：
Sparano JA;Gray RJ;Makower DF;Pritchard KI;Albain KS;Hayes DF;Geyer CE Jr;Dees EC;Goetz MP;Olson JA Jr;Lively T;Badve SS;Saphner TJ;Wagner LI;Whelan TJ;Ellis MJ;Paik S;Wood WC;Ravdin PM;Keane MM;Gomez Moreno HL;Reddy PS;Goggins TF;Mayer IA;Brufsky AM;Toppmeyer DL;Kaklamani VG;Berenberg JL;Abrams J;Sledge GW Jr
通讯作者：
Sledge GW Jr
外泌体：乳腺癌的新兴生物标志物
影响因子：
--
作者：
Jia Y;Chen Y;Wang Q;Jayasinghe U;Luo X;Wei Q;Wang J;Xiong H;Chen C;Xu B;Hu W;Wang L;Zhao W;Zhou J
通讯作者：
Zhou J
影响因子：
7
作者：
Mertins, Philipp;Yang, Feng;Carr, Steven A.
通讯作者：
Carr, Steven A.
影响因子：
--
作者：
Moon PG;Lee JE;Cho YE;Lee SJ;Chae YS;Jung JH;Kim IS;Park HY;Baek MC
通讯作者：
Baek MC