Proteomic Analyses Discern the Developmental Inclusion of Albumin in Pig Enamel: A New Model for Human Enamel Hypomineralization.
Proteomic Analyses Discern the Developmental Inclusion of Albumin in Pig Enamel: A New Model for Human Enamel Hypomineralization.
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DOI：
10.3390/ijms242115577
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发表时间：
2023-10-25
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
釉质中白蛋白过多是普遍存在的牙齿发育缺陷的特征，称为白垩牙或磨牙矿化不足(MH)。本研究使用猪牙的蛋白质组学分析，以辨别发育起源和萌出后污染，并评估其与低矿化人牙釉质的相似性。在这里，目标是解决迫切需要一种动物模型来揭示MH的病因并改进治疗。猪牙釉质在萌出时是白垩质和柔软的，但它很快就会变硬，形成一个坚硬的表面，然后就像人类的牙齿一样，有分界的釉质混浊。对4周龄猪(n=3)和8周龄猪(n=2)以及4例牙釉质混浊的人(n=4)萌出牙、血清和唾液中的釉质进行蛋白质组学分析，结果显示甲胎蛋白(AFP)。甲胎蛋白的表达仅限于产前和围产期发育，其在釉质中的存在表明产前或围产期的包含性。相反，白蛋白在出生后表达，表明出生后牙釉质中含有白蛋白。从牙釉质中提取多肽，经胰酶消化后用纳米液相色谱-串联质谱仪(NanoLC-MS/MS)分析。在所有牙釉质样品中，13种不同的猪AFP独特的胰蛋白酶多肽在所有牙釉质样品中的平均总蛋白数为337个，而在唾液样品中没有。成分、显微硬度和微观结构的相似之处强调了猪模型在揭示MH的病因、白蛋白包涵体的细胞机制和治疗分界性混浊方面的有效性。
Excess albumin in enamel is a characteristic of the prevalent developmental dental defect known as chalky teeth or molar hypomineralization (MH). This study uses proteomic analyses of pig teeth to discern between developmental origin and post-eruptive contamination and to assess the similarity to hypomineralized human enamel. Here, the objective is to address the urgent need for an animal model to uncover the etiology of MH and to improve treatment. Porcine enamel is chalky and soft at eruption; yet, it hardens quickly to form a hard surface and then resembles human teeth with demarcated enamel opacities. Proteomic analyses of enamel from erupted teeth, serum, and saliva from pigs aged 4 (n = 3) and 8 weeks (n = 2) and human (n = 4) molars with demarcated enamel opacities show alpha-fetoprotein (AFP). AFP expression is limited to pre- and perinatal development and its presence in enamel indicates pre- or perinatal inclusion. In contrast, albumin is expressed after birth, indicating postnatal inclusion into enamel. Peptides were extracted from enamel and analyzed by nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) after tryptic digestion. The mean total protein number was 337 in the enamel of all teeth with 13 different unique tryptic peptides of porcine AFP in all enamel samples but none in saliva samples. Similarities in the composition, micro-hardness, and microstructure underscore the usefulness of the porcine model to uncover the MH etiology, cellular mechanisms of albumin inclusion, and treatment for demarcated opacities.
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DOI：
10.1155/2013/684607
发表时间：
2013-09-16
期刊：
ISRN dentistry
影响因子：
--
作者：
Bartlett JD
通讯作者：
Bartlett JD
影响因子：
4
作者：
通讯作者：
--
影响因子：
3.8
作者：
Chay, Pui Ling;Manton, David J.;Palamara, Joseph E. A.
通讯作者：
Palamara, Joseph E. A.
影响因子：
2
作者：
Fagrell, Tobias G.;Dietz, Wolfram;Noren, Jorgen G.
通讯作者：
Noren, Jorgen G.
影响因子：
1.9
作者：
Afzal，Sadaf Haque;Wigen，Tove Irene;Brusevold，Ingvild Johnsen
通讯作者：
Brusevold，Ingvild Johnsen