Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.
Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.
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DOI：
10.1371/journal.pntd.0005902
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发表时间：
2017-09
影响因子：
3.8
通讯作者：
Ritchie SA
中科院分区：
文献类型：
--
作者：
Johnson BJ;Mitchell SN;Paton CJ;Stevenson J;Staunton KM;Snoad N;Beebe N;White BJ;Ritchie SA
最近的兴趣在男性为基础的不育昆虫技术（SIT）和不相容的昆虫技术（IIT），以控制埃及伊蚊和白纹伊蚊种群揭示了需要一个经济，快速的诊断工具，以确定分散和交配成功的不育男性在野外。以前的研究表明，若丹明B是一种巯基活性荧光染料，通过喂糖给药可用于染色昆虫的身体组织和精液。在这里，我们报告的适应这种技术为男性AE。在田间释放期间，允许快速评估竞争力（交配成功率）。在自由飞行期间，通过在50%蜂蜜溶液中喂食0.1、0.2、0.4或0.8%罗丹明B（w/v）的雄性来实现标记。所有浓度均产生>95%的转移到雌性，并在喂食4天后成功产生身体标记，其中0.4%和0.8%溶液产生持续时间最长的身体标记。重要的是，罗丹明B标记对雄性交配竞争力没有影响，并且证明了在野外条件下将标记的精液成功转移到雌性并重新捕获标记的雄性。这些结果表明，罗丹明B是一个潜在的有用的评估方法，男性为基础的SIT/IIT控制策略，以及一个可行的身体标记技术，男性为基础的标记-释放-再捕获实验，没有传统的标记方法的负面副作用。作为用于交配竞争力测定的独立方法，罗丹明B标记比PCR（例如亲子鉴定分析）和稳定同位素精液标记方法便宜，并且比用于评估绝育雄性竞争力的雌性生育力测定耗时更少。最近，以男性为基础的“饲养和释放”策略（释放辐射绝育的男性或携带沃尔巴克氏体的男性），以控制城市伊蚊传播的疾病，包括登革热，基孔肯雅病和寨卡病毒，揭示了需要一种经济和快速的诊断工具，以评估释放的男性与接受女性的野生对应物的交配竞争力。我们的报告详细介绍了男性精液罗丹明B标记的优化和通过喂糖进行的可见身体染色，对生存或交配竞争力没有显着影响，以及原理验证现场发布。这些结果表明，罗丹明B标记是一种经济和快速的评估方法，男性为基础的控制策略，以及一个可行的身体标记技术，男性集中的标记释放-再捕获实验，没有传统的标记方法的负面副作用。使用这种方法有可能使操作人员能够快速、轻松地确定其控制方法的功效，从而使他们能够优化释放，以实现最佳种群抑制。
Recent interest in male-based sterile insect technique (SIT) and incompatible insect technique (IIT) to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success) during field releases. Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v) in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males. These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis) and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males. The recent resurgence in male-based “rear and release” strategies (release of radiation sterilised males or those harbouring Wolbachia) to control diseases transmitted by urban Aedes mosquitoes, including dengue, chikungunya, and Zika, has revealed the need for an economical and rapid diagnostic tool to assess the mating competitiveness of released males against wild counterparts for receptive females. Our report details the optimisation of rhodamine B labelling of male seminal fluid and visible body staining via sugar-feeding with no significant impact on survival or mating competitiveness, as well as proof-of-principle field releases. These results reveal rhodamine B labelling to be an economical and rapid evaluation method for male-based control strategies as well as a viable body marking technique for male focused mark-release-recapture experiments without the negative side-effects of traditional marking methods. The use of this method has the potential to enable operators to determine the efficacy of their control method quickly and easily, allowing them to optimise releases to achieve optimal population suppression.
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影响因子：
3.2
作者：
Dickens BL;Brant HL
通讯作者：
Brant HL
DOI：
10.1186/cc2955
发表时间：
2004-10
期刊：
Critical care (London, England)
影响因子：
--
作者：
Bewick V;Cheek L;Ball J
通讯作者：
Ball J
影响因子：
2.1
作者：
Bellini, Romeo;Albieri, Alessandro;Maini, Stefano
通讯作者：
Maini, Stefano
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
1.9
作者：
FERNANDEZ, GCJ
通讯作者：
FERNANDEZ, GCJ