Gastric tuft cells express DCLK1 and are expanded in hyperplasia.
Gastric tuft cells express DCLK1 and are expanded in hyperplasia.
复制标题
胃簇细胞表达DCLK1并在增生中膨胀。
DOI：
10.1007/s00418-011-0831-1
复制
发表时间：
2011-08
影响因子：
2.3
通讯作者：
Merchant, Juanita L.
中科院分区：
文献类型：
--
作者：
Saqui-Salces, Milena;Keeley, Theresa M.;Grosse, Ann S.;Qiao, Xiaotan T.;El-Zaatari, Mohamad;Gumucio, Deborah L.;Samuelson, Linda C.;Merchant, Juanita L.
上皮簇细胞因其位于细胞顶端的特征性微管束而命名，在细胞顶端暴露于管腔环境。因此，簇细胞存在于多个器官中，包括胃肠道（GI），其中假设顶端“簇”用于检测和传递环境信号。因此，我们的研究目标是在胃肠道发育过程中，然后在正常和化生成人胃的胃丛细胞的特点。分析了来自小鼠胚胎、新生和出生后小鼠的胃肠道。用乙酰化-α-微管蛋白（acTub）抗体检测微管束，免疫组化鉴定簇细胞。另外的簇细胞标记物，例如，双皮质素样激酶1（DCLK 1）用于与acTub共定位。在人胃组织阵列和有或无炎症的小鼠胃中定量簇状细胞。在发育中的肠中，在隐窝和绒毛中的簇状细胞表达E18.5的所有标记物。在胃中，acTub与DCLK 1和其他已建立的簇状细胞标记物共定位于E18.5的胃窦中，但直到出生后第7天才在胃体中，簇状细胞的最高密度聚集在前胃脊处。在增生的人类和小鼠胃中，簇状细胞数量增加。在成人胃肠道中，簇状细胞标志物acTub与DCKL 1和化学感受标志物共表达，TRPM 5.总之，在E18.5时，在胃窦和肠中出现了簇状细胞，但直到断奶后，它们在胃体中的数量才达到最大值。丛细胞数量随着炎症、增生和化生而增加。
Epithelial tuft cells are named for their characteristic microtubule bundle located at the cell apex where it is exposed to the luminal environment. As such, tuft cells are found in multiple organs, including the gastrointestinal (GI) tract where the apical “tuft” is hypothesized to detect and transmit environmental signals. Thus, the goal of our study was to characterize gastric tuft cells during GI tract development, then subsequently in the normal and metaplastic adult stomach. GI tracts from mouse embryos, newborn and post-natal mice were analyzed. Tuft cells were identified by immunohistochemistry using acetylated-α-tubulin (acTub) antibody to detect the microtubule bundle. Additional tuft cell markers, e.g., doublecortin-like kinase 1 (DCLK1), were used to co-localize with acTub. Tuft cells were quantified in human gastric tissue arrays and in mouse stomachs with or without inflammation. In the developing intestine, tuft cells in both the crypts and villi expressed all markers by E18.5. In the stomach, acTub co-localized with DCLK1 and other established tuft cell markers by E18.5 in the antrum but not until postnatal day 7 in the corpus, with the highest density of tuft cells clustered at the forestomach ridge. Tuft cell numbers increased in hyperplastic human and mouse stomachs. In the adult GI tract, the tuft cell marker acTub co-expressed with DCKL1 and chemosensory markers, e.g., TRPM5. In summary, tuft cells appear in the gastric antrum and intestine at E18.5, but their maximal numbers in the corpus are not achieved until after weaning. Tuft cell numbers increase with inflammation, hyperplasia and metaplasia.
登录
查看更多内容
影响因子：
29.4
作者：
Kokrashvili Z;Rodriguez D;Yevshayeva V;Zhou H;Margolskee RF;Mosinger B
通讯作者：
Mosinger B
DOI：
10.1152/ajpgi.1998.274.3.g561
发表时间：
1998-03-01
影响因子：
4.5
作者：
Friis-Hansen, L;Sundler, F;Samuelson, LC
通讯作者：
Samuelson, LC
影响因子：
2.3
作者：
Hass, Nicole;Schwarzenbacher, Karin;Breer, Heinz
通讯作者：
Breer, Heinz
影响因子：
3.6
作者：
ISEKI, S
通讯作者：
ISEKI, S
DOI：
10.1016/j.bbrc.2008.09.031
发表时间：
2008-11-21
影响因子：
3.1
作者：
Kang, Weiqun;Saqui-Salces, Milena;Merchant, Juanita L.
通讯作者：
Merchant, Juanita L.