ESTpass: a web-based server for processing and annotating expressed sequence tag (EST) sequences.
ESTpass: a web-based server for processing and annotating expressed sequence tag (EST) sequences.
复制标题
DOI:
10.1093/nar/gkm369
复制
发表时间:
2007-07
影响因子:
14.9
通讯作者:
Choi YJ
中科院分区:
文献类型:
--
作者:
Lee B;Hong T;Byun SJ;Woo T;Choi YJ
We present a web-based server, called ESTpass, for processing and annotating sequence data from expressed sequence tag (EST) projects. ESTpass accepts a FASTA-formatted EST file and its quality file as inputs, and it then executes a back-end EST analysis pipeline consisting of three consecutive steps. The first is cleansing the input EST sequences. The second is clustering and assembling the cleansed EST sequences using d2_cluster and CAP3 programs and producing putative transcripts. From the CAP3 output, ESTpass detects chimeric EST sequences which are confirmed through comparison with the nr database. The last step is annotating the putative transcript sequences using RefSeq, InterPro, GO and KEGG gene databases according to user-specified options. The major advantages of ESTpass are the integration of cleansing and annotating processes, rigorous chimeric EST detection, exhaustive annotation, and email reporting to inform the user about the progress and to send the analysis results. The ESTpass results include three reports (summary, cleansing and annotation) and download function, as well as graphic statistics. They can be retrieved and downloaded using a standard web browser. The server is available at http://estpass.kobic.re.kr/.
登录
查看更多内容
影响因子:
7
作者:
Burke, J;Davison, D;Hide, W
通讯作者:
Hide, W
影响因子:
14.9
作者:
Min XJ;Butler G;Storms R;Tsang A
通讯作者:
Tsang A
影响因子:
14.9
作者:
Pruitt KD;Tatusova T;Maglott DR
通讯作者:
Maglott DR
影响因子:
14.9
作者:
Maglott D;Ostell J;Pruitt KD;Tatusova T
通讯作者:
Tatusova T
影响因子:
9.5
作者:
Nagaraj, Shivashankar H.;Gasser, Robin B.;Ranganathan, Shoba
通讯作者:
Ranganathan, Shoba