CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis.
CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis.
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CEACAM1 与结直肠肝转移肝切除术后的复发相关。
DOI：
10.1016/j.jss.2017.07.035
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发表时间：
2017
影响因子：
2.2
通讯作者：
Yamaue H.
中科院分区：
文献类型：
--
作者：
Yamaguchi S;Yokoyama S;Ueno M;Hayami S;Mitani Y;Takeuchi A;Shively JE;Yamaue H.
背景癌胚抗原相关细胞粘附分子1（CEACAM 1）在大肠癌的浸润前沿重新表达。转移部位的CEACAM 1表达仍有待研究。目前的研究旨在澄清CEACAM 1表达和肝切除术后复发的结直肠癌肝转移，并解决是否CEACAM 1诱导肿瘤启动属性需要在transmitteds. MethodsImmunohistochemistry分析67例结直肠癌肝转移谁经历了根治性肝切除术。术后复发的危险因素是基于原发肿瘤浸润前沿的CEACAM 1胞质结构域同种型计算的。通过Western blotting检测CD 44和CD 133表达，流式细胞仪检测ALDH 1活性，软琼脂集落形成实验检测CEACAM 1胞浆区长（CEACAM 1-L）和短（CEACAM 1-S）异构体对HT 29和HCT 116大肠癌细胞的影响。CEACAM 1-S在原发肿瘤浸润前沿CEACAM 1-L优势状态下的表达增强是结直肠癌根治性肝切除术后复发的独立因素。CEACAM 1 - 4S转染的HT 29和HCT 116细胞有显着较高的CD 44表达和ALDH 1活性，并增加了生长在锚定非依赖性condition.ConclusionsHigh表达CEACAM 1-S在原发灶浸润前沿与复发和预后的结直肠癌肝转移患者根治性肝切除术后。CEACAM 1 -4S的表达增强了结直肠癌细胞的肿瘤起始特性。
BackgroundCarcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatectomy of colorectal liver metastasis and to address whether CEACAM1 induces tumor-initiating properties needed for growth at metastatic sites.MethodsImmunohistochemical analyses for CEACAM1 were performed in 67 patients with liver metastasis of colorectal cancer who had undergone curative hepatectomy. The risk factors for postoperative recurrence were calculated based on a CEACAM1 cytoplasmic domain isoform at the primary tumor invasion front. To investigate the effects of CEACAM1 cytoplasmic isoforms on HT29 and HCT116 colorectal cancer cells, Western blotting for CD44 and CD133, flow cytometry for ALDH1 activity, and soft-agar colony formation assay were performed.ResultsCEACAM1 long (CEACAM1-L) and short (CEACAM1-S) cytoplasmic domain isoforms are strongly expressed on cancer cells in the liver metastases. Enhanced CEACAM1-S expression in the state of CEACAM1-L dominance at the primary tumor invasion front was an independent factor for colorectal cancer recurrence after curative hepatectomy. CEACAM1-4S-transfected HT29 and HCT116 cells had significantly higher CD44 expression and ALDH1 activity and increased the growth in anchorage-independent condition.ConclusionsHigh expression of CEACAM1-S at the primary lesion invasion front is associated with recurrence and prognosis of patients with colorectal liver metastasis after curative hepatectomy. The expression of CEACAM1-4S enhances the tumor-initiating property of colorectal cancer cells.
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影响因子：
3
作者：
Yamamoto, Naoyuki;Yokoyama, Shozo;Yamaue, Hiroki
通讯作者：
Yamaue, Hiroki
DOI：
10.1083/jcb.108.2.267
发表时间：
1989-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Barnett TR;Kretschmer A;Austen DA;Goebel SJ;Hart JT;Elting JJ;Kamarck ME
通讯作者：
Kamarck ME
CEACAM1长胞质结构域的重新表达
影响因子：
6.4
作者：
Ieda J;Matsuda K;et al
通讯作者：
et al
子宫内膜癌中 CD66a（BGP、C-CAM）（CEA 家族的一种粘附分子）的表达失调。
影响因子：
6
作者：
A. Bamberger;L. Riethdorf;P. Nollau;M. Naumann;I. Erdmann;J. Götze;J. Brümmer;Heinrich M. Schulte;Christoph Wagener;Thomas Löning
通讯作者：
Thomas Löning
DOI：
10.1073/pnas.84.14.4831
发表时间：
1987-07-01
影响因子：
11.1
作者：
GUNNING, P;LEAVITT, J;KEDES, L
通讯作者：
KEDES, L