Identification and gene mapping of a narrow and upper-albino leaf mutant in rice (Oryza sativa L.)

Identification and gene mapping of a narrow and upper-albino leaf mutant in rice (Oryza sativa L.)
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水稻窄叶上部白化突变体的鉴定和基因定位(Oryza sativa L.)

DOI:
10.1007/s11434-012-5154-7
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发表时间:
2012-04
影响因子:
--
通讯作者:
Sang Xianchun
Sang Xianchun
中科院分区:
--
文献类型:
--
作者:
Xu Fangfang;Lin Tingting;He Guanghua;Sang Xianchun

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叶片由颜色和形状性状组成。叶片是水稻光合作用的重要器官,研究叶片发育对提高水稻产量和品质具有重要意义。从用甲基磺酸乙酯处理的籼型恢复系金恢10的后代中鉴定出一个窄叶上部白化突变体nul 1。在田间条件下,突变体显示狭窄和上部白化叶片与显着降低光合色素含量在其整个发展。窄叶性状是由小脉数量减少引起的。与野生型相比,nul 1突变体的生育期延长了约8 d,有效穗数、结实率和千粒重等农艺性状显著下降。遗传分析表明,窄叶和上部白化叶性状表现为共分离,受一对隐性基因控制。Nul 1基因定位在第7号染色体上Indel标记Ind 07 -1和简单重复序列标记RM 21637之间。标记之间的物理距离为75 kb,并根据水稻日本晴基因组序列在该区域注释了8个基因。这些结果为Nul 1基因的克隆和功能研究奠定了基础。
The leaf blade consists of color and shape traits. Studies of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis. A narrow and upper-albino leaf mutant (nul1) was identified from among progeny of the indica restorer line Jinhui10 raised from seeds treated with ethyl methane sulfonate. Under field conditions, the mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents throughout their development. The narrow-leaf trait is caused by a decreased number of small veins. In contrast to the wild type, the growth period was extended by approximately 8 d and agronomic traits, such as effective panicle number, percentage seed set and 1000-grain weight, declined significantly in the nul1 mutant. Genetic analysis suggested that the narrow and upper-albino leaf characteristics showed coseparation and were controlled by one recessive gene. The Nul1 gene was mapped onto chromosome 7 between the Indel marker Ind07-1 and the Simple Sequence Repeat marker RM21637. The physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome sequence. These results provide a foundation for cloning and function analysis of Nul1.
水稻窄叶突变体的遗传分析和基因定位(Oryza sativa L.)
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