Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)
Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)
复制标题
水稻窄叶突变体的遗传分析和基因定位（Oryza sativa L.）
DOI：
10.1007/s11434-009-0098-2
复制
发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Li SuJuan
中科院分区：
文献类型：
--
作者：
Wang DeKai;Tao YueZhi;Li KeLei;Liu HeQin;Li SuJuan
以中华11号水稻为材料，经T-DNA转化获得窄叶突变体，表现出半矮化、花期延迟、叶片窄内卷、结实率低等形态异常特征。突变体旗叶的净光合作用速率（饱和光下）显着低于野生型。此外，灌浆期突变体旗叶的叶片蒸腾速率和气孔导度均低于野生型。结果发现突变表型不是由T-DNA插入引起的。遗传分析表明，该突变体由单个隐性基因控制，称为 nal3(t)。利用源自 nal3(t) 与籼稻品种龙特富 B 杂交的大型 F2 作图群体构建了遗传连锁图谱，该群体在 12 号染色体上通过 BAS 方法从 366 个 SSR 标记中鉴定出 6 个多态性标记。基因 nal3(t) 被定位在标记 RM7018 和 RM3331 之间。根据日本晴和 93-11 之间 nal3(t) 所在区域的序列多样性，利用 22 个新开发的 STS 标记对 nal3(t) 位点进行精细定位，最终将 nal3(t) 定位到 STS 标记 NS10 和 RH12-8 之间的 136 kb 区域。
A narrow leaf mutant was obtained after T-DNA transformation conducted on a rice variety Zhonghua 11. Several abnormal morphological characteristics, including semi-dwarf, delayed flowering time, narrow and inward rolling leaves, and lower seed-setting, were observed. The rate of net photosynthesis (under saturate light) of flag leaves in the mutant was significantly lower than that of the wild type. Moreover, the leaf transpiration rate and stomatal conductance in the mutant flag leaf were lower than those of the wild type at the grain filling stage. It was found that the mutant phenotype was not caused by the T-DNA insertion. Genetic analysis showed that the mutant was controlled by a single recessive gene, designated as nal3(t). A genetic linkage map was constructed using a large F2 mapping population derived from a cross between nal3(t) and an indica variety Longtefu B with 6 polymorphic markers on chromosome 12 identified from 366 SSR markers by the BAS method. Gene nal3(t) was mapped between the markers RM7018 and RM3331. Fine mapping of nal3(t) locus was conducted with 22 newly developed STS markers based on the sequence diversity around the region harboring nal3(t) between Nipponbare and 93–11, and nal3(t) was finally mapped to a 136-kb region between the STS markers NS10 and RH12-8.
登录
查看更多内容
DOI：
10.1007/bf02172406
发表时间：
1996-10
期刊：
Molecular and General Genetics MGG
影响因子：
--
作者：
O. Panaud;X. Chen;S. McCouch
通讯作者：
O. Panaud;X. Chen;S. McCouch
影响因子：
--
作者：
Changjie Yan;Song Yan;Zhengqiu Zhang;G. Liang;Jufei Lu;M. Gu
通讯作者：
Changjie Yan;Song Yan;Zhengqiu Zhang;G. Liang;Jufei Lu;M. Gu
影响因子：
7.4
作者：
Micol, JL;Hake, S
通讯作者：
Hake, S
影响因子：
11.4
作者：
Mallory, AC;Reinhart, BJ;Bartel, DP
通讯作者：
Bartel, DP
DOI：
--
发表时间：
2003-12
期刊：
Shi yan sheng wu xue bao
影响因子：
--
作者：
G. Shen;Xin Qi Wang;L. Yin;Jiang Wang;Lin Li;Jing Zhang
通讯作者：
G. Shen;Xin Qi Wang;L. Yin;Jiang Wang;Lin Li;Jing Zhang