Epigallocatechin gallate reduces vascular inflammation in db/db mice possibly through an NF-κB-mediated mechanism.
Epigallocatechin gallate reduces vascular inflammation in db/db mice possibly through an NF-κB-mediated mechanism.
复制标题
DOI：
10.1002/mnfr.201200040
复制
发表时间：
2012-09
影响因子：
5.2
通讯作者：
Liu, Dongmin
中科院分区：
文献类型：
--
作者：
Babu, Pon V. Anandh;Si, Hongwei;Liu, Dongmin
高血糖诱导的血管炎症导致单核细胞粘附内皮是糖尿病动脉粥样硬化发病的关键事件。我们研究了绿茶中发现的一种主要儿茶素——表没食子儿茶素没食子酸酯（EGCG）是否能减少糖尿病患者的血管炎症。用绿茶儿茶素预处理人主动脉内皮细胞（HAEC），然后再加入高糖（25 mM） 72小时。EGCG在生理可达到的浓度（1µM）下，在静态和流动条件下均能显著抑制高糖诱导的单核细胞对HAEC的粘附。EGCG还降低了内皮细胞中nfκ b调控的转录活性。6周龄糖尿病db/db小鼠分别饲喂含有0%或0.1% EGCG的日粮8周。从db/db、db/db- egcg和对照db/+小鼠的主动脉血管中分离ECs。补充EGCG极大地抑制了糖尿病增加的单核细胞对内皮细胞的粘附，这与降低循环趋化因子水平有关，并减少了db/db-EGCG小鼠主动脉内皮细胞的趋化因子和粘附分子的分泌。EGCG处理降低了db/db-EGCG小鼠主动脉血管中NFκB p65的核易位，降低了血压和血清胆固醇和甘油三酯浓度。EGCG可能对糖尿病的血管炎症有直接的保护作用。
Hyperglycemia-induced vascular inflammation resulting in the adhesion of monocytes to endothelium is a key event in the pathogenesis of atherosclerosis in diabetes. We investigated whether epigallocatechin gallate (EGCG), a major catechin found in green tea, reduces vascular inflammation in diabetes. Human aortic endothelial cells (HAEC) were pre-treated with green tea catechins before the addition of high glucose (25 mM) for 72 h. EGCG at physiologically achievable concentration (1 µM) significantly inhibited high glucose-induced adhesion of monocytes to HAEC both in static and under flow conditions. EGCG also reduced NFκB-regulated transcriptional activity in ECs. Six-week-old diabetic db/db mice were fed a diet containing 0% or 0.1% EGCG for 8 wk. ECs were isolated from aortic vessels of db/db, db/db-EGCG, and control db/+ mice. EGCG supplementation greatly suppressed diabetes-increased monocytes adhesion to ECs, which is associated with reduced circulating levels of chemokines, and reduced secretions of chemokines and adhesion molecules by aortic ECs from db/db-EGCG mice. EGCG treatment reduced nuclear translocation of NFκB p65 in aortic vessels, decreased blood pressure and serum concentrations of cholesterol and triglycerides in db/db-EGCG mice. EGCG may have a direct protective effect against vascular inflammation in diabetes.
登录
查看更多内容
影响因子：
5.4
作者：
Pittet, Mikael J.;Swirski, Filip K.
通讯作者：
Swirski, Filip K.
DOI：
10.1093/jn/138.9.1677
发表时间：
2008-09
期刊：
The Journal of nutrition
影响因子：
--
作者：
Bose M;Lambert JD;Ju J;Reuhl KR;Shapses SA;Yang CS
通讯作者：
Yang CS
DOI：
10.1161/01.atv.zhq0504.2260
发表时间：
2004-05-01
影响因子：
8.7
作者：
Srinivasan, S;Hatley, ME;Hedrick, CC
通讯作者：
Hedrick, CC
影响因子：
4.2
作者：
Lambert, JD;Hong, JG;Yang, CS
通讯作者：
Yang, CS
影响因子：
1.7
作者：
Nishiyama, Tatsuaki;Mishima, Kenji;Saito, Ichiro
通讯作者：
Saito, Ichiro