Functional properties of CD8(+) lymphocytes in patients with pleural plaque and malignant mesothelioma.
Functional properties of CD8(+) lymphocytes in patients with pleural plaque and malignant mesothelioma.
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DOI：
10.1155/2014/670140
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发表时间：
2014
影响因子：
4.1
通讯作者：
Otsuki T
中科院分区：
文献类型：
--
作者：
Kumagai-Takei N;Nishimura Y;Maeda M;Hayashi H;Matsuzaki H;Lee S;Kishimoto T;Fukuoka K;Nakano T;Otsuki T
已知石棉暴露可引起恶性间皮瘤（MM），并且CD 8 + T细胞在抗肿瘤免疫中起关键作用。我们研究了石棉暴露患者胸膜斑块（PL）和MM外周血CD 8+淋巴细胞的属性。三组之间的PBMC中的CD 3 + CD 8+细胞的百分比没有差异，虽然PL和MM组的PBMC总数低于健康志愿者（HV）。PMA/Ionomycin刺激的CD 8+淋巴细胞中IFN-γ +和CD 107 a+细胞的百分比在三组之间没有差异。PL组和MM组新鲜CD 8+淋巴细胞中穿孔素+细胞和CD 45 RA −细胞的数量高于HV组。PL组PMA/ionomycin刺激的CD 8+淋巴细胞中颗粒酶B+和穿孔素+细胞的数量高于HV组。与PL患者相比，MM组在刺激后显示CD 8+淋巴细胞中穿孔素水平降低。这些结果表明，MM患者的外周血CD 8+淋巴细胞的刺激诱导的细胞毒性损伤的特点，PL和MM患者有一个共同的特点，这些淋巴细胞的功能改变，即记忆细胞的增加，可能与接触石棉。
It is known that asbestos exposure can cause malignant mesothelioma (MM) and that CD8+ T cells play a critical role in antitumor immunity. We examined the properties of peripheral blood CD8+ lymphocytes from asbestos-exposed patients with pleural plaque (PL) and MM. The percentage of CD3+CD8+ cells in PBMCs did not differ among the three groups, although the total numbers of PBMCs of the PL and MM groups were lower than those of the healthy volunteers (HV). The percentage of IFN-γ + and CD107a+ cells in PMA/ionomycin-stimulated CD8+ lymphocytes did not differ among the three groups. Percentages of perforin+ cells and CD45RA− cells in fresh CD8+ lymphocytes of PL and MM groups were higher than those of HV. Percentages of granzyme B+ and perforin+ cells in PMA/ionomycin-stimulated CD8+ lymphocytes were higher in PL group compared with HV. The MM group showed a decrease of perforin level in CD8+ lymphocytes after stimulation compared with patients with PL. These results indicate that MM patients have characteristics of impairment in stimulation-induced cytotoxicity of peripheral blood CD8+ lymphocytes and that PL and MM patients have a common character of functional alteration in those lymphocytes, namely, an increase in memory cells, possibly related to exposure to asbestos.
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DOI：
10.1177/039463200902200304
发表时间：
2009-07-01
影响因子：
3.5
作者：
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影响因子：
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