Concepts and principles of photodynamic therapy as an alternative antifungal discovery platform.
Concepts and principles of photodynamic therapy as an alternative antifungal discovery platform.
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DOI：
10.3389/fmicb.2012.00120
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发表时间：
2012
影响因子：
5.2
通讯作者：
Tegos GP
中科院分区：
文献类型：
--
作者：
Dai T;Fuchs BB;Coleman JJ;Prates RA;Astrakas C;St Denis TG;Ribeiro MS;Mylonakis E;Hamblin MR;Tegos GP
寄生性真菌病原体可引起浅表或严重的侵袭性感染，特别是在免疫功能低下和虚弱的患者中。侵袭性真菌病对人类健康的威胁呈指数增长，这是由于诊断缓慢和存在相对较少的可用和有效的抗真菌药物。因此，全身性真菌感染导致高归因死亡率。迫切需要寻求和部署新的有效的替代抗真菌对策。光动力疗法（PDT）被确立为恶性肿瘤和年龄相关性黄斑变性的成功模式，但光动力灭活只是最近才被广泛研究，作为一种替代的抗菌剂发现和开发平台。光动力灭活的概念需要微生物暴露于外源性或内源性光敏剂分子，随后是可见光能量，通常是红色/近红外区域的波长，其引起光敏剂的激发，导致产生单线态氧和其他活性氧物质，其与细胞内组分反应，并因此产生细胞灭活和死亡。抗真菌PDT是一个越来越受关注的领域，因为研究正在推进（i）确定光灭活中涉及的光化学和生物物理机制;（ii）开发有效的和临床相容的光敏剂;（iii）了解光灭活如何受到关键微生物表型元素多药耐药性和外排、毒力和致病决定因素以及生物膜形成的影响;（iv）探索新的光敏剂输送平台;及（v）确定临床环境以外的光灭活应用，例如环境消毒剂。
Opportunistic fungal pathogens may cause superficial or serious invasive infections, especially in immunocompromised and debilitated patients. Invasive mycoses represent an exponentially growing threat for human health due to a combination of slow diagnosis and the existence of relatively few classes of available and effective antifungal drugs. Therefore systemic fungal infections result in high attributable mortality. There is an urgent need to pursue and deploy novel and effective alternative antifungal countermeasures. Photodynamic therapy (PDT) was established as a successful modality for malignancies and age-related macular degeneration but photodynamic inactivation has only recently been intensively investigated as an alternative antimicrobial discovery and development platform. The concept of photodynamic inactivation requires microbial exposure to either exogenous or endogenous photosensitizer molecules, followed by visible light energy, typically wavelengths in the red/near infrared region that cause the excitation of the photosensitizers resulting in the production of singlet oxygen and other reactive oxygen species that react with intracellular components, and consequently produce cell inactivation and death. Antifungal PDT is an area of increasing interest, as research is advancing (i) to identify the photochemical and photophysical mechanisms involved in photoinactivation; (ii) to develop potent and clinically compatible photosensitizers; (iii) to understand how photoinactivation is affected by key microbial phenotypic elements multidrug resistance and efflux, virulence and pathogenesis determinants, and formation of biofilms; (iv) to explore novel photosensitizer delivery platforms; and (v) to identify photoinactivation applications beyond the clinical setting such as environmental disinfectants.
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影响因子：
4.9
作者：
Dai, Tianhong;de Arce, Vida J. Bil;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
DOI：
10.1007/978-1-60761-697-9_13
发表时间：
2010-01-01
期刊：
PHOTODYNAMIC THERAPY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Biel, Merrill A.
通讯作者：
Biel, Merrill A.
影响因子：
3.1
作者：
Bisland, SK;Chien, C;Burch, S
通讯作者：
Burch, S
DOI：
10.1016/s0924-8579(08)70009-8
发表时间：
2008-11-01
影响因子：
10.8
作者：
Caston-Osorio, J. J.;Rivero, A.;Torre-Cisneros, J.
通讯作者：
Torre-Cisneros, J.
影响因子：
4.9
作者：
Bombelli, Cecilia;Bordi, Federico;Venanzi, Mariano
通讯作者：
Venanzi, Mariano