Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration.

Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration.
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生长素参与角蕨配子体分生组织再生。

DOI:
10.3390/ijms242115832
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发表时间:
2023-10-31
影响因子:
5.6
通讯作者:
--
中科院分区:
生物学2区
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--
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水蕨属两性配子体的生长发育依赖于边缘分生组织的细胞增殖,当边缘分生组织被破坏时,它将在体缘的新位置再生。我们建立了一种激光消融方法来破坏分生组织中的单个初始细胞。消融导致细胞增殖停止,同时生长素合成基因(CrTAA 2)和细胞增殖标记基因(CrWOXB)的表达消失。新的分生组织再生发生在距离最初消融后两天的可预测距离内,这通过细胞增殖和CrTAA 2的表达来标志。天然生长素吲哚-3-乙酸(IAA),合成生长素2,4-二氯苯氧乙酸(2,4-D),或运输抑制剂naphthylphthalamic acid(NPA)的治疗改变了定位的原始边缘分生组织向顶端的配子体。IAA在破坏原始分生组织后改变了再生分生组织的位置。生长素参与定位的边缘分生组织在水蕨的模型,以涵盖这些结果。
Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (CrTAA2) and a cell proliferation marker gene (CrWOXB). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of CrTAA2. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results.
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