The defense response in Arabidopsis thaliana against Fusarium sporotrichioides.
The defense response in Arabidopsis thaliana against Fusarium sporotrichioides.
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DOI：
10.1186/1477-5956-10-61
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发表时间：
2012-10-30
期刊：
影响因子：
2
通讯作者：
Nishiuchi T
中科院分区：
文献类型：
--
作者：
Asano T;Kimura M;Nishiuchi T
某些禾本科植物，如玉米和小麦是镰刀菌的寄主；然而，寄主植物与孢子毛线虫之间的分子相互作用尚不清楚。也不知道拟南芥和孢子毛线虫之间是否会发生相互作用。为了理解这些相互作用，我们进行了蛋白质组学分析。以拟南芥叶片和花接种孢子毛线虫。接种孢子毛线虫可增加拟南芥植物防御素1.2 （PDF1.2）和致病相关蛋白1 (PR1) mRNA的积累。此外，在拟南芥中代表MAP激酶的丝裂原活化蛋白激酶3 （MPK3）和丝裂原活化蛋白激酶6 （MPK6）通过接种孢子毛状芽孢杆菌被激活。蛋白质组学分析显示，接种孢子毛线虫后，部分防御相关蛋白表达上调，而光合和代谢相关蛋白表达下调。对接种禾枯菌后的上调蛋白进行了蛋白质组学分析。接种禾草假丝酵母侵染的拟南芥叶片后，GSTF4和GSTF7等谷胱甘肽s -转移酶（GSTs）表达上调。另一方面，GSTF3和GSTF9通过接种孢子毛线虫而特异上调。这些结果表明拟南芥是孢子毛线虫的寄主植物。我们发现拟南芥的防御反应是由孢子毛线虫感染引发的。
Certain graminaceous plants such as Zea mays and Triticum aestivum serve as hosts for Fusarium sporotrichioides; however, molecular interactions between the host plants and F. sporotrichioides remain unknown. It is also not known whether any interaction between Arabidopsis thaliana and F. sporotrichioides can occur. To understand these interactions, we performed proteomic analysis. Arabidopsis leaves and flowers were inoculated with F. sporotrichioides. Accumulation of PLANT DEFENSIN1.2 (PDF1.2) and PATHOGENESIS RELATED1 (PR1) mRNA in Arabidopsis were increased by inoculation of F. sporotrichioides. Furthermore, mitogen-activated protein kinase 3 (MPK3) and mitogen-activated protein kinase 6 (MPK6), which represent MAP kinases in Arabidopsis, were activated by inoculation of F. sporotrichioides. Proteomic analysis revealed that some defense-related proteins were upregulated, while the expression of photosynthesis- and metabolism-related proteins was down regulated, by inoculation with F. sporotrichioides. We carried out the proteomic analysis about upregulated proteins by inoculation with Fusarium graminearum. The glutathione S-transferases (GSTs), such as GSTF4 and GSTF7 were upregulated, by inoculation with F. graminearum-infected Arabidopsis leaves. On the other hand, GSTF3 and GSTF9 were uniquely upregulated, by inoculation with F. sporotrichioides. These results indicate that Arabidopsis is a host plant for F. sporotrichioides. We revealed that defense response of Arabidopsis is initiated by infection with F. sporotrichioides.
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影响因子：
6.9
作者：
Chivasa, S;Hamilton, JM;Slabas, AR
通讯作者：
Slabas, AR
影响因子：
4.4
作者：
Igawa, Tomoko;Takahashi-Ando, Naoko;Kimura, Makoto
通讯作者：
Kimura, Makoto
影响因子：
5.5
作者：
Bai, GH;Desjardins, AE;Plattner, RD
通讯作者：
Plattner, RD
影响因子：
3.5
作者：
Makandar, R;Essig, JS;Shah, J
通讯作者：
Shah, J
DOI：
10.1094/mpmi-23-7-0861
发表时间：
2010-07
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Makandar R;Nalam V;Chaturvedi R;Jeannotte R;Sparks AA;Shah J
通讯作者：
Shah J