Changes in Northern Elephant Seal Skeletal Muscle Following Thirty Days of Fasting and Reduced Activity.
Changes in Northern Elephant Seal Skeletal Muscle Following Thirty Days of Fasting and Reduced Activity.
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DOI：
10.3389/fphys.2020.564555
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发表时间：
2020
影响因子：
4
通讯作者：
Sheffield-Moore M
中科院分区：
文献类型：
--
作者：
Wright TJ;Davis RW;Holser RR;Hückstädt LA;Danesi CP;Porter C;Widen SG;Williams TM;Costa DP;Sheffield-Moore M
北象海豹（NES，Mirounga angustirostris）每年都会经历一次换毛，在此期间，它们会在陆地上禁食约 40 天，活动减少，体重会减少约四分之一。在刻板的陆地哺乳动物模型中，活动和肌肉负荷的减少导致肌肉质量以及产生力量和有氧代谢的能力下降。然而，禁食女性 NES 中损失的大部分体重来自脂肪，而肌肉质量则在很大程度上得以保留。尽管肌肉质量得以保留，但代谢和收缩能力的潜在变化尚不清楚。为了评估蜕皮期间 NES 骨骼肌的潜在变化，我们在蜕皮前和蜕皮结束约 30 天后收集了 6 只成年雌性 NES 的肌肉活检。使用高分辨率呼吸测量法评估骨骼肌的呼吸能力，并提取 RNA 以评估基因表达的变化。尽管一个月的活动减少、禁食和体重减轻，但骨骼肌呼吸能力得以保留，而 OXPHOS 呼吸能力没有变化。蜕皮与 162 个上调基因相关，其中包括那些有利于脂质代谢的基因。我们鉴定了 172 个下调基因，包括编码核糖体蛋白的基因以及与骨骼肌力转导和葡萄糖代谢相关的基因。蜕皮 30 天后，NES 骨骼肌代谢能力得以保留，尽管机械传导可能受到损害。在没有运动刺激的情况下，禁食引起的肌肉代谢变化可能会刺激与保持慢氧化肌肉的质量和代谢能力相关的途径。
Northern elephant seals (NES, Mirounga angustirostris) undergo an annual molt during which they spend ∼40 days fasting on land with reduced activity and lose approximately one-quarter of their body mass. Reduced activity and muscle load in stereotypic terrestrial mammalian models results in decreased muscle mass and capacity for force production and aerobic metabolism. However, the majority of lost mass in fasting female NES is from fat while muscle mass is largely preserved. Although muscle mass is preserved, potential changes to the metabolic and contractile capacity are unknown. To assess potential changes in NES skeletal muscle during molt, we collected muscle biopsies from 6 adult female NES before the molt and after ∼30 days at the end of the molt. Skeletal muscle was assessed for respiratory capacity using high resolution respirometry, and RNA was extracted to assess changes in gene expression. Despite a month of reduced activity, fasting, and weight loss, skeletal muscle respiratory capacity was preserved with no change in OXPHOS respiratory capacity. Molt was associated with 162 upregulated genes including those favoring lipid metabolism. We identified 172 downregulated genes including those coding for ribosomal proteins and genes associated with skeletal muscle force transduction and glucose metabolism. Following ∼30 days of molt, NES skeletal muscle metabolic capacity is preserved although mechanotransduction may be compromised. In the absence of exercise stimulus, fasting-induced shifts in muscle metabolism may stimulate pathways associated with preserving the mass and metabolic capacity of slow oxidative muscle.
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影响因子：
8.1
作者：
Dyar KA;Ciciliot S;Wright LE;Biensø RS;Tagliazucchi GM;Patel VR;Forcato M;Paz MI;Gudiksen A;Solagna F;Albiero M;Moretti I;Eckel-Mahan KL;Baldi P;Sassone-Corsi P;Rizzuto R;Bicciato S;Pilegaard H;Blaauw B;Schiaffino S
通讯作者：
Schiaffino S
DOI：
10.1086/physzool.68.6.30163799
发表时间：
1995-11-01
期刊：
PHYSIOLOGICAL ZOOLOGY
影响因子：
--
作者：
BOILY, P;LAVIGNE, DM
通讯作者：
LAVIGNE, DM
影响因子：
4.3
作者：
Morales PE;Bucarey JL;Espinosa A
通讯作者：
Espinosa A
影响因子：
4
作者：
Busiello RA;Savarese S;Lombardi A
通讯作者：
Lombardi A
影响因子：
14.9
作者：
Edgar, R;Domrachev, M;Lash, AE
通讯作者：
Lash, AE