Recovery of Memory B-cell Subsets and Persistence of Antibodies in Convalescent COVID-19 Patients.
Recovery of Memory B-cell Subsets and Persistence of Antibodies in Convalescent COVID-19 Patients.
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DOI：
10.4269/ajtmh.21-0883
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发表时间：
2021-09-27
期刊：
影响因子：
--
通讯作者：
Babu S
中科院分区：
文献类型：
--
作者：
Rajamanickam A;Kumar NP;Nancy P A;Selvaraj N;Munisankar S;Renji RM;V V;Murhekar M;Thangaraj JWV;Kumar MS;Kumar CPG;Bhatnagar T;Ponnaiah M;Sabarinathan R;Kumar VS;Babu S
研究由严重急性呼吸综合征冠状病毒-2 （SARS-CoV-2）感染引起的防御性免疫反应的持续时间至关重要。我们检测了7组COVID-19患者的SARS-CoV-2特异性抗体反应和体外记忆b细胞亚群，这些患者根据反转录聚合酶链反应确认SARS-CoV-2感染的天数进行分类。我们的数据显示，IgG和中和抗体的水平从第15天到第30天到第61天到第90天开始上升，此后趋于平稳。幼稚B细胞和非典型记忆B细胞的出现频率从第15 ~ 30天到第61 ~ 90天下降，之后趋于平稳。相比之下，未成熟B细胞、经典记忆B细胞、激活记忆B细胞和浆细胞的频率在第15 ~ 30天、第61 ~ 90天增加，之后趋于平稳。与轻症患者相比，重症患者表现出初始细胞、非典型记忆B细胞和激活记忆B细胞的频率增加，未成熟B细胞、中枢记忆B细胞和浆细胞的频率降低。因此，我们的数据表明，在COVID-19恢复期个体中，记忆b细胞亚群频率发生改变，体液免疫持续存在。
It is essential to examine the longevity of the defensive immune response engendered by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. We examined the SARS-CoV-2-specific antibody responses and ex vivo memory B-cell subsets in seven groups of individuals with COVID-19 classified based on days since reverse-transcription polymerase chain reaction confirmation of SARS-CoV-2 infection. Our data showed that the levels of IgG and neutralizing antibodies started increasing from days 15 to 30 to days 61 to 90, and plateaued thereafter. The frequencies of naive B cells and atypical memory B cells decreased from days 15 to 30 to days 61 to 90, and plateaued thereafter. In contrast, the frequencies of immature B cells, classical memory B cells, activated memory B cells, and plasma cells increased from days 15 to 30 to days 61 to 90, and plateaued thereafter. Patients with severe COVID-19 exhibited increased frequencies of naive cells, atypical memory B cells, and activated memory B cells, and lower frequencies of immature B cells, central memory B cells, and plasma cells when compared with patients with mild COVID-19. Therefore, our data suggest modifications in memory B-cell subset frequencies and persistence of humoral immunity in convalescent individuals with COVID-19.
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影响因子：
64.8
作者：
Gaebler C;Wang Z;Lorenzi JCC;Muecksch F;Finkin S;Tokuyama M;Cho A;Jankovic M;Schaefer-Babajew D;Oliveira TY;Cipolla M;Viant C;Barnes CO;Bram Y;Breton G;Hägglöf T;Mendoza P;Hurley A;Turroja M;Gordon K;Millard KG;Ramos V;Schmidt F;Weisblum Y;Jha D;Tankelevich M;Martinez-Delgado G;Yee J;Patel R;Dizon J;Unson-O'Brien C;Shimeliovich I;Robbiani DF;Zhao Z;Gazumyan A;Schwartz RE;Hatziioannou T;Bjorkman PJ;Mehandru S;Bieniasz PD;Caskey M;Nussenzweig MC
通讯作者：
Nussenzweig MC
IGA主导对SARS-COV-2的早期中和抗体反应。
影响因子：
17.1
作者：
Sterlin D;Mathian A;Miyara M;Mohr A;Anna F;Claër L;Quentric P;Fadlallah J;Devilliers H;Ghillani P;Gunn C;Hockett R;Mudumba S;Guihot A;Luyt CE;Mayaux J;Beurton A;Fourati S;Bruel T;Schwartz O;Lacorte JM;Yssel H;Parizot C;Dorgham K;Charneau P;Amoura Z;Gorochov G
通讯作者：
Gorochov G
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
15.3
作者：
Moir, Susan;Ho, Jason;Fauci, Anthony S.
通讯作者：
Fauci, Anthony S.