Target of rapamycin signaling orchestrates growth-defense trade-offs in plants.
Target of rapamycin signaling orchestrates growth-defense trade-offs in plants.
复制标题
DOI：
10.1111/nph.14785
复制
发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Hofte M
中科院分区：
文献类型：
--
作者：
De Vleesschauwer D;Filipe O;Hoffman G;Seifi HS;Haeck A;Canlas P;Van Bockhaven J;De Waele E;Demeestere K;Ronald P;Hofte M
植物对微生物病原体的防御往往伴随着显著的生长抑制。植物如何将免疫系统功能与正常生长和发育结合起来，目前还知之甚少。在这里，我们研究了雷帕霉素靶标(TOR)，一种进化保守的丝氨酸/苏氨酸激酶，在植物防御反应中的作用。我们以水稻为模型系统，综合运用了化学分析、遗传分析、基因组分析和细胞分析。我们证明了TOR和Raptor(mTOR的调节相关蛋白)的异位表达对水稻的生长发育具有正向调节作用。对TOR特异性抑制剂雷帕霉素处理的水稻细胞进行的转录组分析表明，TOR不仅决定了参与中枢和次生代谢、细胞周期和转录的广泛基因的转录重新编程，而且还抑制了许多与防御相关的基因。Tor高表达品系表现出对细菌和真菌病原菌的敏感性增加，而Tor信号降低的植株表现出更强的抗性。最后，我们发现TOR拮抗经典防御激素水杨酸和茉莉酸的作用。综上所述，这些结果表明，TOR作为一种分子开关，以牺牲细胞免疫为代价，激活了细胞增殖和植物生长。
Plant defense to microbial pathogens is often accompanied by significant growth inhibition. How plants merge immune system function with normal growth and development is still poorly understood. Here, we investigated the role of target of rapamycin (TOR), an evolutionary conserved serine/threonine kinase, in the plant defense response. We used rice as a model system and applied a combination of chemical, genetic, genomic and cell-based analyses. We demonstrate that ectopic expression of TOR and Raptor (regulatory-associated protein of mTOR), a protein previously demonstrated to interact with TOR in Arabidopsis, positively regulates growth and development in rice. Transcriptome analysis of rice cells treated with the TOR-specific inhibitor rapamycin revealed that TOR not only dictates transcriptional reprogramming of extensive gene sets involved in central and secondary metabolism, cell cycle and transcription, but also suppresses many defense-related genes. TOR overexpression lines displayed increased susceptibility to both bacterial and fungal pathogens, whereas plants with reduced TOR signaling displayed enhanced resistance. Finally, we found that TOR antagonizes the action of the classic defense hormones salicylic acid and jasmonic acid. Together these results indicate that TOR acts as a molecular switch for activation of cell proliferation and plant growth at the expense of cellular immunity.
登录
查看更多内容
DOI：
10.1126/science.1215327
发表时间：
2012-05-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hawley SA;Fullerton MD;Ross FA;Schertzer JD;Chevtzoff C;Walker KJ;Peggie MW;Zibrova D;Green KA;Mustard KJ;Kemp BE;Sakamoto K;Steinberg GR;Hardie DG
通讯作者：
Hardie DG
影响因子：
9.2
作者：
Kapahi, P;Zid, BM;Benzer, S
通讯作者：
Benzer, S
影响因子：
7.4
作者：
De Vleesschauwer, David;Seifi, Hamed Soren;Hofte, Monica
通讯作者：
Hofte, Monica
影响因子：
4.6
作者：
Bakshi A;Moin M;Kumar MU;Reddy AB;Ren M;Datla R;Siddiq EA;Kirti PB
通讯作者：
Kirti PB
影响因子：
5.6
作者：
De Vleesschauwer D;Xu J;Höfte M
通讯作者：
Höfte M