Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits.
Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits.
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新西兰兔骨骼肌发育中miRNA的动态变化
DOI：
10.1186/s12864-021-07896-5
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发表时间：
2021-07-27
期刊：
影响因子：
4.4
通讯作者：
Ling Y
中科院分区：
文献类型：
--
作者：
Jing J;Jiang X;Zhu C;Zheng Q;Ji Q;Yin H;Huang J;Zhu Y;Wang J;Qin S;Ling Y
研究背景miRNA是调控骨骼肌发育的复杂动态网络中的关键分子之一。新西兰兔从胎儿到成年骨骼肌miRNAs的景观尚未被揭示yet.ResultsIn this study，9个RNA-seq文库的胎儿，儿童和成年兔的腿部肌肉构建。共鉴定出278个差异表达的miRNA（DEmiRNAs）。在胎儿组和儿童组中，主要的功能富集涉及膜和转运。DEmiRNAs表达上调与骨骼肌的分化和肥大有关，表达下调与骨骼肌的结构和代谢能力有关。在儿童与成人组中，功能与定位和运输相关，通路与ECM、肌肉结构和肥大相关。最后，在这两组动物中发现了靶基因最多的ocu-miR-185- 3 p和ocu-miR-370- 3 p为hub-miRNAs。此外，还探讨了miRNAs在两个连续阶段的潜在功能变化。其中，选择靶基因最多的ocu-miR-185- 3 p和ocu-miR-370- 3 p作为hub-miRNA。这些数据丰富了对肉兔发育调控分子的认识，为肉兔的分子育种提供了新的视角。
BackgroundmiRNA is one of the crucial roles in the complex and dynamic network that regulates the development of skeletal muscle. The landscape of skeletal muscle miRNAs from fetus to adult in New Zealand rabbits has not been revealed yet.ResultsIn this study, nine RNA-seq libraries of fetus, child and adult rabbits’ leg muscles were constructed. A total of 278 differentially expressed miRNAs (DEmiRNAs) were identified. In the fetus vs. child group, the main functional enrichments were involved in membrane and transport. Pathway enriched terms of up-regulated DEmiRNAs were connected with the differentiation and hypertrophy of skeletal muscle, and down-regulated ones were related to muscle structure and metabolic capacity. In the child vs. adult group, functions were associated to positioning and transportation, and pathways were relevant to ECM, muscle structure and hypertrophy. Finally, ocu-miR-185-3p and ocu-miR-370-3p, which had the most target genes, were identified as hub-miRNAs in these two groups.ConclusionsIn short, we summarized the highly expressed and uniquely expressed DEmiRNAs of fetus, child and adult rabbits’ leg muscles. Besides, the potential functional changes of miRNAs in two consecutive stages have been explored. Among them, the ocu-miR-185-3p and ocu-miR-370-3p with the most target genes were selected as hub-miRNAs. These data improved the understanding of the regulatory molecules of meat rabbit development, and provided a novel perspective for molecular breeding of meat rabbits.
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DOI：
10.1093/dnares/dsx022
发表时间：
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期刊：
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