Serum SELENBP1 and VCL Are Effective Biomarkers for Clinical and Forensic Diagnosis of Coronary Artery Spasm.
Serum SELENBP1 and VCL Are Effective Biomarkers for Clinical and Forensic Diagnosis of Coronary Artery Spasm.
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血清Selenbp1和VCL是用于冠状动脉痉挛的临床和法医诊断的有效生物标志物。
DOI：
10.3390/ijms232113266
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发表时间：
2022-10-31
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
冠状动脉痉挛(CAS)在许多缺血性心脏实体的发病机制中起着重要作用；然而，在临床和法医环境下，CAS尚无明确的诊断生物标志物。本研究旨在通过建立兔CAS激发模型，并结合定量血清蛋白质组学、平行反应监测/质谱靶向蛋白质组学和偏最小二乘判别分析(偏最小二乘判别分析)来识别此类血清生物标志物。我们的结果表明，SELENBP1和VCL是CAS的潜在候选生物标志物。在独立的临床样本中，CAS患者血清中SELENBP1和VCL的水平显著降低，但血细胞中的SELENBP1和VCL水平没有明显下降，其原因可能是心肌细胞分泌减少。受试者工作特征(ROC)分析曲线下面积SELENBP1为0.9384，VCL为0.9180。血清SELENBP1和VCL每增加10个单位，CAS风险分别降低32.3%和53.6%。在法医标本中，单独检测血清SELENBP1诊断CAS死亡的敏感性为100.0%，特异性为72.73%，与VCL联合诊断的特异性为100.0%，优于传统的cTnI和CK-MB生物标志物。因此，血清SELENBP1和VCL可作为CAS临床和法医学诊断的有效生物标志物。
Coronary artery spasm (CAS) plays an important role in the pathogenesis of many ischemic heart entities; however, there are no established diagnostic biomarkers for CAS in clinical and forensic settings. This present study aimed to identify such serum biomarkers by establishing a rabbit CAS provocation model and integrating quantitative serum proteomics, parallel reaction monitoring/mass spectrometry-based targeted proteomics, and partial least-squares discriminant analysis (PLS-DA). Our results suggested that SELENBP1 and VCL were potential candidate biomarkers for CAS. In independent clinical samples, SELENBP1 and VCL were validated to be significantly lower in serum but not blood cells from CAS patients, with the reasons for this possibly due to the decreased secretion from cardiomyocytes. The areas under the curve of the receiver operating characteristics (ROC) analysis were 0.9384 for SELENBP1 and 0.9180 for VCL when diagnosing CAS. The CAS risk decreased by 32.3% and 53.6% for every 10 unit increases in the serum SELENBP1 and VCL, respectively. In forensic samples, serum SELENBP1 alone diagnosed CAS-induced deaths at a sensitivity of 100.0% and specificity of 72.73%, and its combination with VCL yielded a diagnostic specificity of 100.0%, which was superior to the traditional biomarkers of cTnI and CK-MB. Therefore, serum SELENBP1 and VCL could be effective biomarkers for both the clinical and forensic diagnosis of CAS.
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影响因子：
2.8
作者：
Hung, Ming-Jui;Cherng, Wen-Jin;Li, Li-Fu
通讯作者：
Li, Li-Fu
DOI：
10.1016/s0735-1097(85)80221-7
发表时间：
1985-01-01
影响因子：
24
作者：
FACTOR, SM;CHO, S
通讯作者：
CHO, S
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
20.1
作者：
Hayashi M;Shimizu W;Albert CM
通讯作者：
Albert CM
影响因子：
3.6
作者：
Hung MJ;Hu P;Hung MY
通讯作者：
Hung MY