Injectable calcium phosphate with hydrogel fibers encapsulating induced pluripotent, dental pulp and bone marrow stem cells for bone repair.
Injectable calcium phosphate with hydrogel fibers encapsulating induced pluripotent, dental pulp and bone marrow stem cells for bone repair.
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可注射磷酸钙，水凝胶纤维封装诱导多能、牙髓和骨髓干细胞，用于骨修复
DOI：
10.1016/j.msec.2016.08.019
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发表时间：
2016-12-01
期刊：
影响因子：
--
通讯作者：
Xu HH
中科院分区：
文献类型：
--
作者：
Wang L;Zhang C;Li C;Weir MD;Wang P;Reynolds MA;Zhao L;Xu HH
人诱导多能干细胞衍生的间充质干细胞（hiPSC-MSCs）、牙髓干细胞（hDPSC）和骨髓间充质干细胞（hBMSCs）是再生医学中令人兴奋的细胞来源。然而，还没有报道比较hDPSC、hBMSC和hiPSC-MSC在可注射磷酸钙骨水泥（CPC）支架中用于骨工程的情况。本研究的目的是：（1）开发一种新型的可注射CPC，其含有包封干细胞的水凝胶纤维;（2）首次比较hDPSC、来自骨髓的hiPSC-MSC（BM-hiPSC-MSC）和来自包皮的hiPSC-MSC（FS-hiPSC-MSC）以及hBMSC在CPC中的细胞活力、增殖和成骨分化。结果表明，注射液不损害细胞活力。可注射CPC的孔隙率为62%。所有四种类型的细胞在CPC中的水凝胶纤维内增殖和分化成骨谱系。hDPSC、BM-hiPSC-MSC和hBMSC表现出高碱性磷酸酶、runt相关转录因子、I型胶原和骨钙素基因表达。细胞合成的矿物质随时间增加（p < 0.05），在hDPSC、BM-hiPSC-MSC和hBMSC之间没有显著差异（p > 0.1）。CPC内的hDPSC、BM-hiPSC-MSC和hBMSC在14 d时的矿化是1 d时的14倍。FS-hiPSC-MSC与其他细胞相比在成骨分化方面较差。总之，hDPSC、BM-hiPSC-MSC和hBMSC在骨组织工程中具有相似的前景;然而，FS-hiPSC-MSC在成骨方面相对较差。具有细胞包封水凝胶纤维的新型可注射CPC可增强牙科、颅面和骨科应用中的骨再生。
Human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs), dental pulp stem cells (hDPSCs) and bone marrow MSCs (hBMSCs) are exciting cell sources in regenerative medicine. However, there has been no report comparing hDPSCs, hBMSCs and hiPSC-MSCs for bone engineering in an injectable calcium phosphate cement (CPC) scaffold. The objectives of this study were to: (1) develop a novel injectable CPC containing hydrogel fibers encapsulating stem cells for bone engineering, and (2) compare cell viability, proliferation and osteogenic differentiation of hDPSCs, hiPSC-MSCs from bone marrow (BM-hiPSC-MSCs) and from foreskin (FS-hiPSC-MSCs), and hBMSCs in CPC for the first time. The results showed that the injection did not harm cell viability. The porosity of injectable CPC was 62%. All four types of cells proliferated and differentiated down the osteogenic lineage inside hydrogel fibers in CPC. hDPSCs, BM-hiPSC-MSCs, and hBMSCs exhibited high alkaline phosphatase, runt-related transcription factor, collagen I, and osteocalcin gene expressions. Cell-synthesized minerals increased with time (p < 0.05), with no significant difference among hDPSCs, BM-hiPSC-MSCs and hBMSCs (p > 0.1). Mineralization by hDPSCs, BM-hiPSC-MSCs, and hBMSCs inside CPC at 14 d was 14-fold that at 1 d. FS-hiPSC-MSCs were inferior in osteogenic differentiation compared to the other cells. In conclusion, hDPSCs, BM-hiPSC-MSCs and hBMSCs are similarly and highly promising for bone tissue engineering; however, FS-hiPSC-MSCs were relatively inferior in osteogenesis. The novel injectable CPC with cell-encapsulating hydrogel fibers may enhance bone regeneration in dental, craniofacial and orthopedic applications.
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影响因子：
14
作者：
Carey, LE;Xu, HHK;Chow, LC
通讯作者：
Chow, LC
影响因子：
8.8
作者：
Hong SG;Winkler T;Wu C;Guo V;Pittaluga S;Nicolae A;Donahue RE;Metzger ME;Price SD;Uchida N;Kuznetsov SA;Kilts T;Li L;Robey PG;Dunbar CE
通讯作者：
Dunbar CE
影响因子：
14
作者：
Kumar, Girish;Tison, Christopher K.;Chatterjee, Kaushik;Pine, P. Scott;McDaniel, Jennifer H.;Salit, Marc L.;Young, Marian F.;Simon, Carl G., Jr.
通讯作者：
Simon, Carl G., Jr.
影响因子：
5.2
作者：
Baksh, Dolores;Yao, Raphael;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
影响因子：
4
作者：
Hynes, Kim;Menicanin, Danijela;Bartold, P. Mark
通讯作者：
Bartold, P. Mark