The determination of stem cell fate by 3D scaffold structures through the control of cell shape.
The determination of stem cell fate by 3D scaffold structures through the control of cell shape.
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DOI：
10.1016/j.biomaterials.2011.08.054
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发表时间：
2011-12
期刊：
影响因子：
14
通讯作者：
Simon, Carl G., Jr.
中科院分区：
文献类型：
--
作者：
Kumar, Girish;Tison, Christopher K.;Chatterjee, Kaushik;Pine, P. Scott;McDaniel, Jennifer H.;Salit, Marc L.;Young, Marian F.;Simon, Carl G., Jr.
研究了干细胞对具有不同3D结构的支架库的响应。微阵列筛选显示，每种类型的支架结构诱导一个独特的基因表达签名在原代人骨髓基质细胞（hBMSCs）。层次聚类分析表明，治疗分类的支架结构，而不是由高分子化学表明，支架结构比支架组合物的影响。此外，支架结构对hBMSC功能的影响由细胞形状介导。在所有测试的支架中，只有具有纳米纤维形态的支架能够在没有成骨补充剂的情况下驱动hBMSC向下成骨谱系。纳米纤维支架迫使hBMSCs呈现细长的高度分支的形态。在成骨对照中观察到相同的形态，其中hBMSC在成骨补充剂（OS）存在下在平坦聚合物膜上培养。相比之下，在没有OS的情况下，在平坦的聚合物膜上培养的hBMSCs呈现更圆和更少分支的形态。这些结果表明，细胞对支架结构比以前认识到的更敏感，并表明支架功效可以通过定制支架结构以迫使细胞形成引导它们向下分化所需谱系的形态来优化。
Stem cell response to a library of scaffolds with varied 3D structures was investigated. Microarray screening revealed that each type of scaffold structure induced a unique gene expression signature in primary human bone marrow stromal cells (hBMSCs). Hierarchical cluster analysis showed that treatments sorted by scaffold structure and not by polymer chemistry suggesting that scaffold structure was more influential than scaffold composition. Further, the effects of scaffold structure on hBMSC function were mediated by cell shape. Of all the scaffolds tested, only scaffolds with a nanofibrous morphology were able to drive the hBMSCs down an osteogenic lineage in the absence of osteogenic supplements. Nanofiber scaffolds forced the hBMSCs to assume an elongated, highly branched morphology. This same morphology was seen in osteogenic controls where hBMSCs were cultured on flat polymer films in the presence of osteogenic supplements (OS). In contrast, hBMSCs cultured on flat polymer films in the absence of OS assumed a more rounded and less-branched morphology. These results indicate that cells are more sensitive to scaffold structure than previously appreciated and suggest that scaffold efficacy can be optimized by tailoring the scaffold structure to force cells into morphologies that direct them to differentiate down the desired lineage.
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影响因子：
14
作者：
Smith, Laura A.;Liu, Xiaohua;Hu, Jiang;Ma, Peter X.
通讯作者：
Ma, Peter X.
DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
4
作者：
Rodríguez, JP;González, M;Cambiazo, V
通讯作者：
Cambiazo, V
影响因子：
3.6
作者：
Abrams, GA;Goodman, SL;Murphy, CJ
通讯作者：
Murphy, CJ
DOI：
10.1002/jbm.b.30050
发表时间：
2004-08-15
影响因子：
3.4
作者：
Darling, AL;Sun, W
通讯作者：
Sun, W