Effects of maternal smoking on the placental expression of genes related to angiogenesis and apoptosis during the first trimester.
Effects of maternal smoking on the placental expression of genes related to angiogenesis and apoptosis during the first trimester.
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DOI：
10.1371/journal.pone.0106140
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sekizawa A
中科院分区：
文献类型：
--
作者：
Kawashima A;Koide K;Ventura W;Hori K;Takenaka S;Maruyama D;Matsuoka R;Ichizuka K;Sekizawa A
据报道，母亲吸烟与流产、胎儿生长受限和胎盘早剥有关，而且与先兆子痫风险降低有关。在本研究中，我们调查了在妊娠早期绒毛组织的基因表达水平。我们比较了吸烟和非吸烟孕妇绒毛组织中血管生成和凋亡相关基因的表达水平。我们收集了57例妊娠6-8周因非医学原因要求手术终止妊娠的妇女的绒毛组织样本。通过血清可替宁水平评估母亲吸烟状况，并根据血清可替宁水平将患者分为主动吸烟者和非吸烟者。采用真实的时间PCR定量检测胎盘组织中VEGFA、PGF、FLT 1、HIF 1A、TP 53、BAX和BCL 2 mRNA水平。吸烟组PGF和HIF 1A基因表达水平显著高于非吸烟组。我们没有观察到组间VEGFA或FLT 1表达的任何显著差异。吸烟组TP 53和BAX基因表达水平显著高于非吸烟组。吸烟组BAX/BCL 2 mRNA比值显著高于非吸烟组。我们的研究结果表明，吸烟可能会影响胎盘在怀孕早期。孕早期母亲吸烟可能与绒毛缺氧有关，这可能影响血管生成和细胞凋亡。
Maternal cigarette smoking is reportedly associated with miscarriage, fetal growth restriction and placental abruption, and is paradoxically associated with a decreased risk of developing preeclampsia. In the present study, we investigated the gene expression levels of villous tissues in early gestation. We compared the expression levels of the genes related to angiogenesis and apoptosis in the villous tissues obtained from smoking and non-smoking pregnant women. We collected villous tissue samples from 57 women requesting surgical termination due to non-medical reasons at 6–8 weeks of gestation. The maternal cigarette smoking status was evaluated by the level of serum cotinine and patients were divided into active smokers and non-smokers by the serum cotinine level. The placental levels of VEGFA, PGF, FLT1, HIF1A, TP53, BAX and BCL2 mRNA were quantified by real time PCR. The gene expression level of PGF and HIF1A in the active smoker group was significantly higher than that in the non-smoker group. We did not observe any significant differences in the VEGFA or FLT1 expression between the groups. In active smoker group, the gene expression levels of TP53 and BAX were significantly higher than those in the non-smoker group. The ratio of BAX/BCL2 mRNA in the active smoker group was significantly higher than that in the non-smoker group. Our findings revealed that smoking might affect the placenta during early pregnancy. Maternal cigarette smoking in early pregnancy may be associated with villus hypoxia, which may influence angiogenesis and apoptosis.
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DOI：
10.1111/j.1471-0528.2002.01322.x
发表时间：
2002-08-01
影响因子：
5.8
作者：
Jacqz-Aigrain, E;Zhang, D;Oury, JF
通讯作者：
Oury, JF
影响因子：
5.5
作者：
Chen, Baosheng;Longtine, Mark S.;Nelson, D. Michael
通讯作者：
Nelson, D. Michael
影响因子：
4.3
作者：
ASMUSSEN, I
通讯作者：
ASMUSSEN, I
影响因子：
4.8
作者：
Humphrey, Rachel G.;Sonnenberg-Hirche, Christina;Nelson, D. Michael
通讯作者：
Nelson, D. Michael
影响因子：
3.7
作者：
Hung TH;Chen SF;Lo LM;Li MJ;Yeh YL;Hsieh TT
通讯作者：
Hsieh TT