Antioxidant diet protects against emphysema, but increases mortality in cigarette smoke-exposed mice.
Antioxidant diet protects against emphysema, but increases mortality in cigarette smoke-exposed mice.
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DOI：
10.3109/15412555.2011.600361
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发表时间：
2011-10
期刊：
影响因子：
2.2
通讯作者：
Seagrave J
中科院分区：
文献类型：
--
作者：
Nyunoya T;March TH;Tesfaigzi Y;Seagrave J
氧化应激在香烟引起的肺部炎症和肺气肿中起重要作用。我们在8604 Teklad啮齿动物饮食（一种标准的啮齿动物饮食）中添加了冻干水果和蔬菜以及其他补充剂，从而制作了一种营养丰富的饮食。在这项研究中，我们研究了富含抗氧化剂的饮食对香烟引起的肺部炎症和肺气肿的影响。CH3/HeN小鼠分别饲喂常规饮食和抗氧化饮食。这些小鼠分别暴露于过滤空气、低浓度香烟烟雾（总颗粒物：100 mg/m3）或高浓度香烟烟雾（总颗粒物：250mg/m3）中，每天6小时，每周5天，共16周。令人惊讶的是，与摄入常规饮食的高浓度香烟烟雾暴露小鼠（13%）相比，摄入抗氧化剂饮食的高浓度香烟烟雾暴露小鼠的死亡率增加了53%。尸检分析显示，在抗氧化剂饮食中暴露于香烟烟雾的小鼠中，鼻腔阻塞是由于粘液堵塞造成的。然而，与常规饮食/高浓度香烟烟雾对照组相比，抗氧化饮食显著减少了高浓度香烟烟雾暴露小鼠的中性粒细胞炎症和肺气肿。抗氧化饮食不影响支气管肺泡液的抗氧化能力或肺组织的氧化损伤。Pro-MMP-2、MMP-2和MMP-9活性与AOD对香烟烟雾引起的肺气肿的保护作用无关。这些数据表明，抗氧化饮食减少了香烟引起的炎症和肺气肿，但增加了专用鼻子呼吸的小鼠的死亡率。
Oxidative stress plays an important role in cigarette smoke-induced lung inflammation and emphysema. We produced an enriched diet by adding freeze-dried fruits and vegetables and additional supplements to the 8604 Teklad Rodent Diet, a standard rodent diet. In this study, we examined the effects of the antioxidant-enriched diet on cigarette smoke-induced lung inflammation and emphysema. CH3/HeN mice were fed either a regular diet or an antioxidant diet. These mice were exposed to filtered air, a low concentration of cigarette smoke (total particulate matter: 100 mg/m3) or a high concentration of cigarette smoke (total particulate matter: 250mg/m3) for 6 hours/day, 5 days/week for total 16 weeks. Surprisingly, increased mortality (53%) was observed in the high concentration of cigarette smoke-exposed mice fed the antioxidant diet compared to the high concentration of cigarette smoke-exposed mice that were fed a regular diet (13%). The necropsy analysis revealed nasal passage obstruction due to mucous plugging in cigarette smoke-exposed mice on the antioxidant diet. However, the antioxidant diet significantly reduced neutrophilic inflammation and emphysema in the high concentration of cigarette smoke-exposed mice as compared to the regular diet /high concentration of cigarette smoke controls. The antioxidant capacity in the bronchoalveolar fluid or oxidative damage to the lung tissue was not affected by the antioxidant diet. Pro-MMP-2, MMP-2, and MMP-9 activity did not correlate with the protective effects of AOD on cigarette smoke-induced emphysema. These data suggest that the antioxidant diet reduced cigarette smoke -induced inflammation and emphysema, but increased mortality in the obligate nose-breathing mice.
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影响因子：
1.7
作者：
Seagrave, J;Barr, EB;Nikula, KJ
通讯作者：
Nikula, KJ
COPD中的抗氧化治疗进展。
影响因子：
4.3
作者：
Rahman I
通讯作者：
Rahman I
DOI：
10.1152/ajpregu.00343.2007
发表时间：
2007-09-01
影响因子：
2.8
作者：
Borzone, Gisella;Liberona, Leonel;Lisboa, Carmen
通讯作者：
Lisboa, Carmen
DOI：
10.1164/rccm.200506-850oc
发表时间：
2006-03-15
影响因子：
24.7
作者：
Foronjy, RF;Mirochnitchenko, O;D'Armiento, JM
通讯作者：
D'Armiento, JM
影响因子：
9.6
作者：
Rennard, SI
通讯作者：
Rennard, SI