Streptococcus salivarius urease expression: involvement of the phosphoenolpyruvate:sugar phosphotransferase system.

Streptococcus salivarius urease expression: involvement of the phosphoenolpyruvate:sugar phosphotransferase system.
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唾液链球菌脲酶表达:磷酸烯醇丙酮酸:糖磷酸转移酶系统的参与。

DOI:
10.1111/j.1574-6968.1998.tb13135.x
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发表时间:
1998
影响因子:
2.1
通讯作者:
Burne,RA
Burne,RA
中科院分区:
生物学4区
文献类型:
--
作者:
Chen,YY;Hall,TH;Burne,RA

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唾液链球菌57.I中的尿素酶表达受酸性pH诱导,并且在高生长速率和过量碳水化合物下进一步增强。值得注意的是,在相同条件下,磷酸烯醇丙酮酸(PEP):糖磷酸转移酶系统(PTS)活性在口腔链球菌中受到抑制。为了检验PTS可能参与脲酶调节的假设,分离了对2-脱氧葡萄糖具有抗性的自发突变体(PTS-1和PTS-4)。当与野生型相比时,PTS-1缺乏酶IIAManH(EIIAManH)并合成少量的EIIAManL,而PTS-4缺乏EIIAManL,但产生野生型水平的EIIAManH。在稳态下,在连续恒化器培养物中检查尿素酶表达。在碳水化合物限制条件下,在两种突变体中仍然观察到酸性pH的诱导,但与野生型相比,其水平较低。相反,PTS-4的较低表达水平可以在过量碳水化合物中克服。这些数据表明PTS、糖代谢和脲酶表达调节之间存在分子联系。
Urease expression inStreptococcus salivarius57.I is induced by acidic pH, and further enhanced at high growth rate and with excess carbohydrate. Notably, the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) activity is repressed in oral streptococci under the same conditions. To test the hypothesis that the PTS may be involved in urease regulation, spontaneous mutants (PTS-1 and PTS-4) that were resistant to 2-deoxyglucose were isolated. When compared to wild-type, PTS-1 was devoid of enzyme IIAManH(EIIAManH) and synthesized low amounts of EIIAManL, and PTS-4 was devoid of EIIAManL, but produced wild-type levels of EIIAManH. Urease expression was examined in continuous chemostat cultures at steady state. Induction by acidic pH was still observed in both mutants, but at lower levels compared to wild-type, under carbohydrate limiting conditions. Conversely, the lower level of expression in PTS-4 could be overcome in excess carbohydrate. The data indicated evidence of a molecular link between the PTS, sugar metabolism, and regulation of urease expression.
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