Streptococcus salivarius urease expression: involvement of the phosphoenolpyruvate:sugar phosphotransferase system.
Streptococcus salivarius urease expression: involvement of the phosphoenolpyruvate:sugar phosphotransferase system.
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唾液链球菌脲酶表达:磷酸烯醇丙酮酸:糖磷酸转移酶系统的参与。
DOI:
10.1111/j.1574-6968.1998.tb13135.x
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发表时间:
1998
影响因子:
2.1
通讯作者:
Burne,RA
中科院分区:
文献类型:
--
作者:
Chen,YY;Hall,TH;Burne,RA
Urease expression inStreptococcus salivarius57.I is induced by acidic pH, and further enhanced at high growth rate and with excess carbohydrate. Notably, the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) activity is repressed in oral streptococci under the same conditions. To test the hypothesis that the PTS may be involved in urease regulation, spontaneous mutants (PTS-1 and PTS-4) that were resistant to 2-deoxyglucose were isolated. When compared to wild-type, PTS-1 was devoid of enzyme IIAManH(EIIAManH) and synthesized low amounts of EIIAManL, and PTS-4 was devoid of EIIAManL, but produced wild-type levels of EIIAManH. Urease expression was examined in continuous chemostat cultures at steady state. Induction by acidic pH was still observed in both mutants, but at lower levels compared to wild-type, under carbohydrate limiting conditions. Conversely, the lower level of expression in PTS-4 could be overcome in excess carbohydrate. The data indicated evidence of a molecular link between the PTS, sugar metabolism, and regulation of urease expression.
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影响因子:
--
作者:
GAUTHIER, L;BOURASSA, S;VADEBONCOEUR, C
通讯作者:
VADEBONCOEUR, C
影响因子:
2.1
作者:
Chen,YY;Burne,RA
通讯作者:
Burne,RA
影响因子:
--
作者:
S. Bourassa;L. Gauthier;R. Giguère;C. Vadeboncoeur
通讯作者:
C. Vadeboncoeur
影响因子:
3.5
作者:
G. Gagnon;C. Vadeboncoeur;M. Frenette
通讯作者:
M. Frenette
影响因子:
3
作者:
C. Sissons;E. M. Hancock;Hiran Perinpanayagam;T. Cutress
通讯作者:
T. Cutress