The influence of RGD-bearing hydrogels on the re-expression of contractile vascular smooth muscle cell phenotype.
The influence of RGD-bearing hydrogels on the re-expression of contractile vascular smooth muscle cell phenotype.
复制标题
DOI：
10.1016/j.biomaterials.2009.04.038
复制
发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Marchant, Roger E.
中科院分区：
文献类型：
--
作者：
Beamish, Jeffrey A.;Fu, Alexander Y.;Choi, Ae-jin;Haq, Nada A.;Kottke-Marchant, Kandice;Marchant, Roger E.
本研究报道了聚乙二醇二丙烯酸酯(PEGDA)含整合素结合GRGDSP多肽(RGD-Gel)的水凝胶支架(RGD-Gel)支持培养的血管平滑肌细胞(SMCs)向收缩表型再分化的能力。将人冠状动脉SMCs接种于RGD凝胶、含其他细胞外基质衍生多肽、纤维连接蛋白(FN)和层粘连蛋白(LN)的水凝胶上。在含有可溶性肝素的低血清培养基上诱导分化，并通过收缩平滑肌标志物α-肌动蛋白、降钙素和SM-22α的基因表达、蛋白表达和细胞内蛋白组织来监测分化状态。RGD-Gel支持SMC标记基因mRNA的快速诱导(上调2.7-25倍)，其表达水平相当于FN和LN对照。标记物蛋白水平反映了mRNA表达的变化，RGD凝胶上的水平与FN和LN对照没有区别。此外，这些标记物共定位在RGD凝胶上的SMC内的应力纤维中，提示细胞内收缩装置的重现。这些结果表明，在含RGD的水凝胶上培养的SMC可以重新分化为收缩表型，这表明该材料是进一步发展为调节SMC表型的生物活性支架的极佳候选材料。
This study reports on the ability of poly(ethylene glycol) diacrylate (PEGDA) hydrogel scaffolds with pendant integrin-binding GRGDSP peptides (RGD-gels) to support the re-differentiation of cultured vascular smooth muscle cells (SMCs) toward a contractile phenotype. Human coronary SMCs were seeded on RGD-gels, hydrogels with other extracellular matrix derived peptides, fibronectin (FN) and laminin (LN). Differentiation was induced on RGD-gels with low serum medium containing soluble heparin, and the differentiation status was monitored by mRNA expression, protein expression, and intracellular protein organization of the contractile smooth muscle markers, smooth muscle α-actin, calponin and SM-22α. RGD-gels supported a rapid induction (2.7- to 25-fold up-regulation) of SMC marker gene mRNA, with expression levels that were equivalent to FN and LN controls. Marker protein levels mirrored the changes in mRNA expression, with levels on RGD-gels indistinguishable from FN and LN controls. Furthermore, these markers co-localized in stress fibers within SMCs on RGD-gels suggesting the recapitulation of a contractile apparatus within the cells. These results indicate that SMCs cultured on RGD-bearing hydrogels can re-differentiate toward a contractile phenotype suggesting this material is an excellent candidate for further development as a bioactive scaffold that regulates SMC phenotype.
登录
查看更多内容
影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
影响因子：
46.9
作者：
Kim, BS;Nikolovski, J;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
--
作者：
Hwang, Nathaniel S.;Varghese, Shyni;Elisseeff, Jennifer
通讯作者：
Elisseeff, Jennifer
影响因子：
4.8
作者：
Hayashi, K;Saga, H;Sobue, K
通讯作者：
Sobue, K
影响因子：
2.9
作者：
GRAF, J;OGLE, RC;KLEINMAN, HK
通讯作者：
KLEINMAN, HK