Immunogenicity and efficacy of DNA vaccine encoding antigenic AcfA via addition of the molecular adjuvant Myd88 against Vibrio alginolyticus in Epinephelus coioides
Immunogenicity and efficacy of DNA vaccine encoding antigenic AcfA via addition of the molecular adjuvant Myd88 against Vibrio alginolyticus in Epinephelus coioides
复制标题
添加分子佐剂 Myd88 编码抗原 AcfA 的 DNA 疫苗针对斜带石斑鱼溶藻弧菌的免疫原性和功效
DOI：
10.1016/j.fsi.2017.05.021
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发表时间：
2017-07
影响因子：
4.7
通讯作者：
Wu Zaohe
中科院分区：
文献类型：
--
作者：
Huang Yucong;Cai Shuanghu;Pang Huanying;Jian Jichang;Wu Zaohe
DNA疫苗已广泛用于动物微生物感染。近年来，利用分子佐剂提高DNA疫苗的免疫原性的研究越来越多。MyD88是激活信号级联反应并产生炎症介质的适配分子之一，其在哺乳动物中已被证实的免疫作用和佐剂潜力，在鱼类中鲜有报道。本研究构建了质粒pcMyD88，并通过与编码AcfA的DNA疫苗共注射抗橙斑石斑鱼溶藻弧菌感染，探讨了MyD88作为分子佐剂的能力。结果提示，DNA疫苗pcacfA或分子佐剂pcMyD88从注射的肌肉运输到肾脏和脾脏并刺激宿主免疫系统进行后期保护至少需要7天。pcMyD88与DNA疫苗pcacfA共注射可显著提高特异性抗体水平和MHCIIα、MHCIIα、CD4、CD8α、IL-1β、TNFα等免疫相关基因的表达水平。此外，pcMyD88增强了pcacfA对病毒的免疫保护作用。在接种后10周的激射试验中，pcMyD88 + pcacfA组的RPS为83.3%，显著高于单独pcacfA组（73.3%）。综上所述，这些结果清楚地表明MyD88是橙斑石斑鱼DNA疫苗pcacfA的有效佐剂。
DNA vaccines had been widely used against microbial infection in animals. The use of molecular adjuvants to improve the immunogenicity of DNA vaccines has been increasingly studied in recent years. MyD88 is one of the adapter molecules to activate the signaling cascades and produces inflammatory mediators, and its immunological role and adjuvant potential which had been proved in mammals were rarely reported in fish species. In this study, plasmid pcMyD88 was constructed and the capacity of MyD88 as molecular adjuvant was explored by co-injecting with a DNA vaccine encoding AcfA against Vibrio alginolyticus infection in orange spotted grouper. The results suggested that it needed at least 7 days to transported DNA vaccine pcacfA or molecular adjuvant pcMyD88 from the injected muscle to kidney and spleens and stimulate host's immune system for later protection. The co-injection of pcMyD88 with DNA vaccine pcacfA could increase significantly specific antibody levels and the expression levels of the immune-related genes including MHCIα, MHCIIα, CD4, CD8α, IL-1β and TNFα. Furthermore, pcMyD88 enhanced the immunoprotection of pcacfA againstV. alginolyticusinfection, with the significantly higher RPS of 83.3% in pcMyD88 + pcacfA group compared with that of pcacfA alone (73.3%) at challenging test of 10 weeks post vaccination. Together, these results clearly demonstrate that MyD88 is an effective adjuvant for the DNA vaccine pcacfA in orange spotted grouper.
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影响因子：
4.4
作者：
Hølvold LB;Myhr AI;Dalmo RA
通讯作者：
Dalmo RA
南海滨珊瑚病原菌溶藻弧菌的首次鉴定
影响因子：
3.7
作者：
Zhenyu X;Shaowen K;Chaoqun H;Zhixiong Z;Shifeng W;Yongcan Z
通讯作者：
Yongcan Z
影响因子：
2.9
作者：
Apichaya Taechavasonyoo;I. Hirono;H. Kondo
通讯作者：
Apichaya Taechavasonyoo;I. Hirono;H. Kondo
影响因子：
4.5
作者：
Fengrong Zheng;Hongzhan Liu;Xiu-qin Sun;Yongqiang Zhang;Baiyu Zhang;Zhaojun Teng;Hou Yongjiang;Bo Wang
通讯作者：
Fengrong Zheng;Hongzhan Liu;Xiu-qin Sun;Yongqiang Zhang;Baiyu Zhang;Zhaojun Teng;Hou Yongjiang;Bo Wang
影响因子：
5.5
作者：
Martinez-Lopez, A.;Garcia-Valtanen, P.;Estepa, A.
通讯作者：
Estepa, A.