De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica.
De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica.
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产油酵母解脂耶氏酵母中反式 10、顺式 12 共轭亚油酸的从头合成
DOI：
10.1186/1475-2859-11-51
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发表时间：
2012-07-04
影响因子：
6.4
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Zhang B;Rong C;Chen H;Song Y;Zhang H;Chen W
BackgroundConjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA, an effective and isomer-specific production process is required for medicinal and nutritional purposes.ResultsThe linoleic acid isomerase gene from Propionibacterium acnes was expressed in Yarrowia lipolytica Polh. Codon usage optimization of the PAI and multi-copy integration significantly improved the expression level of PAI in Y. lipolytica. The percentage of trans-10, cis-12 CLA was six times higher in yeast carrying the codon-optimized gene than in yeast carrying the native gene. In combination with multi-copy integration, the production yield was raised to approximately 30-fold. The amount of trans-10, cis-12 CLA reached 5.9% of total fatty acid yield in transformed Y. lipolytica.Conclusions
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影响因子：
3
作者：
Kohno-Murase, J;Iwabuchi, M;Imamura, J
通讯作者：
Imamura, J
影响因子：
3
作者：
Deng, Ming-De;Grund, Alan D.;Rosson, Reinhardt A.
通讯作者：
Rosson, Reinhardt A.
影响因子：
1.9
作者：
Haas, MJ;Kramer, JKG;Yurawecz, MP
通讯作者：
Yurawecz, MP
影响因子：
6
作者：
Churruca, Itziar;Fernandez-Quintela, Alfredo;Puy Portillo, Maria
通讯作者：
Puy Portillo, Maria
影响因子：
3.2
作者：
Nicaud, JM;Madzak, C;Gaillardin, C
通讯作者：
Gaillardin, C