Lactobacillus saerimneri and Lactobacillus ruminis: novel human-derived probiotic strains with immunomodulatory activities.
Lactobacillus saerimneri and Lactobacillus ruminis: novel human-derived probiotic strains with immunomodulatory activities.
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Saerimneri和Ruminis乳酸乳杆菌：具有免疫调节活性的新型人类源自益生菌菌株。
DOI：
10.1111/j.1574-6968.2009.01506.x
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发表时间：
2009-04
影响因子：
2.1
通讯作者：
Tumwasorn S
中科院分区：
文献类型：
--
作者：
Taweechotipatr M;Iyer C;Spinler JK;Versalovic J;Tumwasorn S
从健康志愿者的粪便样品中分离人源性乳酸杆菌。从不同的志愿者中选择了46株分离株，并对其免疫调节特性进行了研究。评估来自每个分离物的条件培养基对脂多糖（LPS）活化的THP-1单核细胞中肿瘤坏死因子（TNF）产生的影响。在46株乳杆菌中，有12株在不同程度上显著抑制了TNF的产生。乳杆菌菌株TH 58显示出最有效的TNF抑制活性（70%抑制）。相反，乳杆菌菌株TH 14通过激活THP-1单核细胞中的TNF产生而表现出免疫刺激特性。乳酸杆菌TH 14在没有LPS刺激的情况下诱导NF-κB活化，而乳酸杆菌TH 58对NF-κB信号传导没有影响，与LPS刺激无关。经16 S rRNA基因序列分析，菌株TH 58为萨氏乳杆菌（Lactobacillus saerimneri），TH 14为瘤胃乳杆菌（Lactobacillus ruminis）。这是首次从人体分离到L. saerimneri和L. ruminis是人类的固有物种，具有TNF刺激活性。我们的数据表明这两种菌株作为免疫益生菌候选物的潜在用途。
Human-derived lactobacilli were isolated from fecal samples of healthy volunteers. Forty-six isolates from different volunteers were selected and investigated for their immunomodulatory properties. Conditioned medium from each isolate was assessed for its effect on tumor necrosis factor (TNF) production in lipopolysaccharide (LPS) - activated THP-1 monocytes. Of 46 Lactobacillus isolates, 12 significantly inhibited TNF production in varying magnitude. Lactobacillus strain TH58 displayed the most potent TNF - inhibitory activity (70% inhibition). In contrast, Lactobacillus strain TH14 exhibited immunostimulatory property by activating TNF production in THP-1 monocytes. Lactobacillus TH14 induced NF-κB activation in the absence of LPS stimulation, whereas Lactobacillus TH58 had no effect on NF-κB signaling, irrespective of LPS stimulation. Strain TH58 was identified as Lactobacillus saerimneri and strain TH14 as Lactobacillus ruminis by sequence analysis of the16S rRNA gene. This is the first report of a human isolate of L. saerimneri with TNF inhibitory activity and L. ruminis, an indigenous species to humans, with TNF stimulatory activity. Our data suggest the potential use of these two strains as immunoprobiotic candidates.
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影响因子：
4.7
作者：
Perdigón, G;Galdeano, CM;Medici, M
通讯作者：
Medici, M
DOI：
10.1196/annals.1326.009
发表时间：
2006-01-01
期刊：
INFLAMMATORY BOWEL DISEASE: GENETICS, BARRIER FUNCTION, IMMUNOLOGIC MECHANISMS, AND MICROBIAL PATHWAYS
影响因子：
--
作者：
Egan, Laurence J.;Toruner, Murat
通讯作者：
Toruner, Murat
影响因子：
3.5
作者：
Tejada-Simon, MV;Lee, JH;Pestka, JJ
通讯作者：
Pestka, JJ
影响因子：
3.4
作者：
Kim, Sung O.;Sheikh, Haroon I.;Reid, Gregor
通讯作者：
Reid, Gregor
影响因子：
5.5
作者：
Maassen, CBM;van Holten-Neelen, C;Claassen, E
通讯作者：
Claassen, E