Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo.
Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo.
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血小板衍生生长因子导致肺细胞增殖和体内胶原沉积。
DOI：
--
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发表时间：
1996
影响因子：
6
通讯作者：
Thomas R. Ulich
中科院分区：
文献类型：
--
作者：
Eunhee S. Yi;Hyesun Lee;Songmei Yin;Pierre;Ildiko Sarosi;Steve Kaufmann;J. Tarpley;Nai San Wang;Thomas R. Ulich;Thomas R. Ulich
血小板衍生生长因子（PDGF）被认为在肺纤维化的病理生理学中发挥作用。通过苏木精和伊红组织学和5-溴脱氧尿苷掺入评价，在大鼠中单次气管内注射给予重组人PDGF-BB导致注射后第2天和第3天支气管周围和血管周围基质细胞增加。支气管上皮细胞和动脉平滑肌细胞的增殖，虽然不明显的常规组织学检查单独，检测到在第2和第3天增加5-溴脱氧尿苷掺入。注射PDGF后，在外周肺泡实质中观察到5-溴脱氧尿苷标记的轻度增加。增生的支气管周围和血管周围的间充质细胞出现的光镜和超微结构的标准，是成纤维细胞的免疫反应波形蛋白，但阴性的α-平滑肌肌动蛋白和结蛋白。每日气管内注射PDGF-BB 3天引起稍明显的支气管周围和血管周围梭形细胞增殖，伴随胶原沉积，如通过Masson三色染色评估的。PDGF诱导的细胞结构和胶原增加在最后一次PDGF注射后5天内消退。总之，肺内注射PDGF-BB可引起肺间充质细胞和上皮细胞的短暂增殖，并伴有胶原沉积。
Platelet-derived growth factor (PDGF) is postulated to play a role in the pathophysiology of pulmonary fibrosis. Recombinant human PDGF-BB administered as a single intratracheal injection in rats causes an increase in peribronchial and perivascular stromal cells on days 2 and 3 after injection as evaluated by hematoxylin and eosin histology and 5-bromodeoxyuridine incorporation. Proliferation of bronchial epithelial cells and arterial smooth muscle cells, although not evident by routine histological examination alone, is detected on days 2 and 3 by increased 5-bromodeoxyuridine incorporation. A mild increase in 5-bromodeoxyuridine labeling is observed in peripheral alveolar parenchyma after injection of PDGF. The proliferative peribronchial and perivascular mesenchymal cells appear by light microscopic and ultrastructural criteria to be fibroblasts that are immunoreactive for vimentin but negative for alpha-smooth muscle actin and desmin. Daily intratracheal injection of PDGF-BB for 3 days causes a slightly more pronounced peribronchial and perivascular spindle cell proliferation accompanied by collagen deposition as evaluated by Masson's trichrome stain. PDGF-induced increases in cellularity and collagen resolve within 5 days after the last PDGF injection. In conclusion, intratracheal injection of PDGF-BB causes transient proliferation of pulmonary mesenchymal and epithelial cells accompanied by collagen deposition.
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DOI：
10.1161/01.atv.11.5.1223
发表时间：
1991-09-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
AMENTO, EP;EHSANI, N;LIBBY, P
通讯作者：
LIBBY, P
影响因子：
20.1
作者：
MAJESKY, MW;GIACHELLI, CM;SCHWARTZ, SM
通讯作者：
SCHWARTZ, SM
影响因子：
56.9
作者：
HART, CE;FORSTROM, JW;BOWENPOPE, DF
通讯作者：
BOWENPOPE, DF
DOI：
--
发表时间：
1994-07
期刊：
The American journal of pathology
影响因子：
--
作者：
Kai Zhang;M. Rekhter;D. Gordon;S. Phan
通讯作者：
Kai Zhang;M. Rekhter;D. Gordon;S. Phan
影响因子：
20.1
作者：
LINDNER, V;GIACHELLI, CM;REIDY, MA
通讯作者：
REIDY, MA