Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner.
Disruption of Autographa Californica Multiple Nucleopolyhedrovirus ac111 Results in Reduced per os Infectivity in a Host-Dependent Manner.
复制标题
苜蓿银纹夜蛾多重核多角体病毒 ac111 的破坏导致以宿主依赖性方式降低经口感染性
DOI：
10.3390/v10100527
复制
发表时间：
2018-09-27
期刊：
影响因子：
--
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Li S;Li L;Zhao H;Liu W
苜蓿银纹夜蛾核型多角体病毒（Autographa californica multiple nucleopolyhedrovirus，AcMNPV）ac111基因在鳞翅目昆虫杆状病毒中高度保守，其在AcMNPV生活史中的功能尚不清楚。为了研究ac111基因的功能，通过在大肠杆菌中同源重组，构建了ac111基因敲除的AcMNPV（vAc111KO）。病毒生长曲线分析和空斑试验表明ac111的缺失对感染性出芽病毒体的产生没有影响。定量实时聚合酶链反应分析证实，在ac111的情况下，病毒DNA复制不受影响。电子显微镜显示，ac111缺失不影响核衣壳组装，闭塞衍生的病毒体形成，或闭塞衍生的病毒体嵌入闭塞机构。然而，体内生物测定表明，虽然ac111的缺失并不影响AcMNPV在甜菜夜蛾幼虫中的经口感染性，但它导致AcMNPV在粉纹夜蛾幼虫中的感染性降低约5倍，并且vAc111KO比野生型病毒杀死粉纹夜蛾幼虫所需时间长约21 h。综上所述，我们的研究结果表明，虽然ac111是不是必不可少的病毒在体外复制，它起着重要的作用，在经口感染的AcMNPV的宿主依赖性的方式。
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac111 gene is highly conserved in lepidopteran-specific baculoviruses, and its function in the AcMNPV life cycle is still unknown. To investigate the function of ac111, an ac111-knockout AcMNPV (vAc111KO) was constructed through homologous recombination in Escherichia coli. Viral growth curve analysis and plaque assays showed that the deletion of ac111 had no effect on infectious budded virion production. Quantitative real-time polymerase chain reaction analysis confirmed that viral DNA replication was unaffected in the absence of ac111. Electron microscopy revealed that the ac111 deletion did not affect nucleocapsid assembly, occlusion-derived virion formation, or the embedding of occlusion-derived virions into the occlusion bodies. However, in vivo bioassays showed that although the deletion of ac111 did not affect the per os infectivity of AcMNPV in Spodoptera exigua larvae, it led to an approximately five-fold reduction in infectivity of AcMNPV in Trichoplusia ni larvae, and vAc111KO took approximately 21 h longer to kill Trichoplusia ni larvae than the wild-type viruses. Taken together, our results demonstrated that although ac111 is not essential for virus replication in vitro, it plays an important role in the per os infectivity of AcMNPV in a host-dependent manner.
登录
查看更多内容
影响因子：
5.4
作者：
Chen, Yun-Ru;Zhong, Silin;Blissard, Gary W.
通讯作者：
Blissard, Gary W.
影响因子：
3.8
作者：
Harrison, Robert L.;Sparks, Wendy O.;Bonning, Bryony C.
通讯作者：
Bonning, Bryony C.
影响因子：
3.7
作者：
Detvisitsakun, Chanitchote;Cain, Erica L.;Passarelli, A. Lorena
通讯作者：
Passarelli, A. Lorena
影响因子：
2.7
作者：
Liang, Changyong;Lan, Dandan;Chen, Xinwen
通讯作者：
Chen, Xinwen
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.