Young bone marrow Sca-1 cells protect aged retina from ischaemia-reperfusion injury through activation of FGF2.
Young bone marrow Sca-1 cells protect aged retina from ischaemia-reperfusion injury through activation of FGF2.
复制标题
年轻的骨髓 Sca-1 细胞通过激活 FGF2 来保护老化的视网膜免受缺血再灌注损伤。
DOI：
10.1111/jcmm.13905
复制
发表时间：
2018-12
影响因子：
5.3
通讯作者：
Li RK
中科院分区：
文献类型：
--
作者：
Shao Z;Wu J;Du G;Song H;Li SH;He S;Li J;Wu J;Weisel RD;Yuan H;Li RK
视网膜神经节细胞凋亡和视神经变性在老年患者中普遍存在，这可能与骨髓（BM）干细胞数量/功能下降有关，因为这两种器官之间可能存在串扰。视网膜缺血再灌注（I/R）损伤加速了这一病理过程。本研究探讨了年轻的骨髓干细胞是否可以在急性I/R损伤后再生和修复老化的视网膜。将年轻的BM干细胞抗原1阳性（Sca-1+）或Sca-1−细胞移植到致死辐射的老年受体小鼠中，分别产生Sca-1+和Sca-1−嵌合体。将动物圈养3个月，以使年轻的Sca-1细胞在老年小鼠的BM中重新繁殖。然后通过眼内压升高诱导视网膜I/R。伤后7天，Sca-1+比Sca-1-chimaeras更好地保留了视觉功能。更多的Sca-1+细胞比Sca-1-细胞归巢到视网膜，并且在Sca-1+嵌合体中更多的细胞分化成神经胶质细胞和小神经胶质细胞。损伤后，视网膜中的Sca-1+细胞减少宿主细胞凋亡，这与Sca-1+嵌合体中成纤维细胞生长因子2（FGF 2）的高表达相关。年轻的Sca-1+细胞通过FGF 2和Akt信号通路重新填充老年视网膜中的干细胞，并减少急性I/R损伤后的细胞凋亡。
Retinal ganglion cell apoptosis and optic nerve degeneration are prevalent in aged patients, which may be related to the decrease in bone marrow (BM) stem cell number/function because of the possible cross‐talk between the two organs. This pathological process is accelerated by retinal ischaemia‐reperfusion (I/R) injury. This study investigated whether young BM stem cells can regenerate and repair the aged retina after acute I/R injury. Young BM stem cell antigen 1 positive (Sca‐1+) or Sca‐1− cells were transplanted into lethally irradiated aged recipient mice to generate Sca‐1+ and Sca‐1− chimaeras, respectively. The animals were housed for 3 months to allow the young Sca‐1 cells to repopulate in the BM of aged mice. Retinal I/R was then induced by elevation of intraocular pressure. Better preservation of visual function was found in Sca‐1+ than Sca‐1− chimaeras 7 days after injury. More Sca‐1+ cells homed to the retina than Sca‐1− cells and more cells differentiated into glial and microglial cells in the Sca‐1+ chimaeras. After injury, Sca‐1+ cells in the retina reduced host cellular apoptosis, which was associated with higher expression of fibroblast growth factor 2 (FGF2) in the Sca‐1+ chimaeras. Young Sca‐1+ cells repopulated the stem cells in the aged retina and diminished cellular apoptosis after acute I/R injury through FGF2 and Akt signalling pathways.
登录
查看更多内容
影响因子：
64.5
作者：
López-Otín C;Blasco MA;Partridge L;Serrano M;Kroemer G
通讯作者：
Kroemer G
影响因子：
4.4
作者：
Li, Yang;Atmaca-Sonmez, Pelin;Enzmann, Volker
通讯作者：
Enzmann, Volker
影响因子：
1.8
作者：
Abdejalil, J;Hamid, M;Serge, P
通讯作者：
Serge, P
影响因子：
4.4
作者：
Fang, Li;Barber, Alistair J.;Shenberger, Jeffrey S.
通讯作者：
Shenberger, Jeffrey S.
影响因子：
4
作者：
Hurley MM;Gronowicz G;Zhu L;Kuhn LT;Rodner C;Xiao L
通讯作者：
Xiao L