A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens.
A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens.
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DOI：
10.1083/jcb.201404127
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发表时间：
2014-12-08
期刊：
影响因子：
--
通讯作者：
Sargent F
中科院分区：
文献类型：
--
作者：
Hamilton JJ;Marlow VL;Owen RA;Costa Mde A;Guo M;Buchanan G;Chandra G;Trost M;Coulthurst SJ;Palmer T;Stanley-Wall NR;Sargent F
穴蛋白 (ChiW) 和肽链内切酶 (ChiX) 协同作用，作为革兰氏阴性细菌粘质沙雷氏菌用来分泌几丁质分解机制的蛋白质分泌系统的组成部分。病原菌通过分泌效应分子来适应环境并操纵宿主的生物化学。粘质沙雷氏菌是一种与医疗保健获得性感染相关的机会性病原体，并且是一种多产的蛋白质分泌者，包括三种几丁质酶（ChiA、ChiB 和 ChiC）和一种几丁质结合蛋白 (Cbp21)。在这项工作中，遗传、生化和蛋白质组学方法鉴定了分泌所有三种几丁质酶和 Cbp21 所需的基因。基因筛选鉴定出一种穴蛋白样蛋白 (ChiW) 和一种推定的 l-丙氨酰-d-谷氨酸内肽酶 (ChiX)，随后的生化分析证实，两者都是整个几丁质分解机制的非裂解性分泌所必需的，在突变体的后期，几丁质酶的分泌被阻断。此外，活细胞成像实验证明了 chiX 和 chiA 的双峰协调表达，并表明表达 chiA 的细胞仍然存活。据建议，ChiW 和 ChiX 作为革兰氏阴性菌使用的蛋白质分泌系统的组成部分协同运作。
A holin (ChiW) and an endopeptidase (ChiX) operate in tandem as components of a protein secretion system used by the gram-negative bacterium Serratia marcescens to secrete the chitinolytic machinery. Pathogenic bacteria adapt to their environment and manipulate the biochemistry of hosts by secretion of effector molecules. Serratia marcescens is an opportunistic pathogen associated with healthcare-acquired infections and is a prolific secretor of proteins, including three chitinases (ChiA, ChiB, and ChiC) and a chitin binding protein (Cbp21). In this work, genetic, biochemical, and proteomic approaches identified genes that were required for secretion of all three chitinases and Cbp21. A genetic screen identified a holin-like protein (ChiW) and a putative l-alanyl-d-glutamate endopeptidase (ChiX), and subsequent biochemical analyses established that both were required for nonlytic secretion of the entire chitinolytic machinery, with chitinase secretion being blocked at a late stage in the mutants. In addition, live-cell imaging experiments demonstrated bimodal and coordinated expression of chiX and chiA and revealed that cells expressing chiA remained viable. It is proposed that ChiW and ChiX operate in tandem as components of a protein secretion system used by gram-negative bacteria.
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影响因子：
7.7
作者：
Hodak, Helene;Galan, Jorge E.
通讯作者：
Galan, Jorge E.
影响因子：
3.2
作者：
Marlow VL;Porter M;Hobley L;Kiley TB;Swedlow JR;Davidson FA;Stanley-Wall NR
通讯作者：
Stanley-Wall NR
DOI：
10.1038/nrmicro2762
发表时间：
2012-04-02
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.4
作者：
Francetic, O;Belin, D;Pugsley, AP
通讯作者：
Pugsley, AP
影响因子：
4.8
作者：
Gründling, A;Bläsi, U;Young, R
通讯作者：
Young, R